Tassone Gabriella, Fidler Samantha J
Department of Clinical Immunology, PathWest Laboratory Medicine, Royal Perth Hospital, Perth, WA, Australia.
Methods Mol Biol. 2012;882:351-7. doi: 10.1007/978-1-61779-842-9_20.
Spleen and lymph node retrieved post-mortem from deceased organ donors are a rich source of lymphocytes. Storage of lymphocytes separated from these sources can be valuable where post-transplant testing (crossmatching) is required. DNA extraction from stored lymphocytes also allows further genetic testing where required, for example additional HLA typing not performed at the time of transplant for donor-specific antibody monitoring. Methods for the isolation and freezing of such cells is described.
从已故器官捐献者尸检中获取的脾脏和淋巴结是淋巴细胞的丰富来源。在需要进行移植后检测（交叉配型）的情况下，储存从这些来源分离出的淋巴细胞可能很有价值。从储存的淋巴细胞中提取DNA还可以在需要时进行进一步的基因检测，例如在移植时未进行的额外HLA分型，用于供体特异性抗体监测。本文描述了分离和冷冻此类细胞的方法。
