Podwojski Katharina, Stephan Christian, Eisenacher Martin
Bayer Pharma AG, Wuppertal, Germany.
Methods Mol Biol. 2012;893:3-21. doi: 10.1007/978-1-61779-885-6_1.
Mass spectrometry is frequently used in quantitative proteomics to detect differentially regulated proteins. A very important but unfortunately oftentimes neglected part in detecting differential proteins is the statistical analysis. Data from proteomics experiments are usually high-dimensional and hence require profound statistical methods. It is especially important to already correctly design a proteomic experiment before it is conducted in the laboratory. Only this can ensure that the statistical analysis is capable of detecting truly differential proteins afterwards. This chapter thus covers aspects of both statistical planning and the actual analysis of quantitative proteomic experiments.
质谱分析法在定量蛋白质组学中经常用于检测差异表达的蛋白质。在检测差异蛋白质时，一个非常重要但不幸的是常常被忽视的部分是统计分析。蛋白质组学实验的数据通常是高维的，因此需要深入的统计方法。在实验室进行蛋白质组学实验之前就正确地设计实验尤为重要。只有这样才能确保后续的统计分析能够检测到真正差异表达的蛋白质。因此，本章涵盖了定量蛋白质组学实验的统计规划和实际分析两个方面。
