Suresh Kumar Kempahanumakkagari, Pandurangappa Malingappa
Department of Studies in Chemistry, Bangalore University, Central College Campus, Dr. Ambedkar Veedhi, Bangalore 5600 01, India.
ScientificWorldJournal. 2012;2012:837672. doi: 10.1100/2012/837672. Epub 2012 May 1.
A sensitive solvent-free extraction protocol for the quantification of arsenic at trace level has been described. It is based on the reaction of arsenic (V) with molybdate in acidic medium in presence of antimony (III) and ascorbic acid as a reducing agent to form a blue-colored arsenomolybdenum blue complex. The complex has been extracted into surfactant phase using Triton X-114, and its absorbance was measured at 690 nm. The detection limit, working range, and the relative standard deviation were found to be 1 ng mL⁻¹, 10-200 ng mL⁻¹, and 1.2%, respectively. The effect of common ions was studied, and the method has been applied to determine trace levels of As(III) and As(V) from a variety of samples like environmental, biological, and commercially procured chemicals.
本文描述了一种用于痕量砷定量分析的灵敏的无溶剂萃取方法。该方法基于在酸性介质中，砷（V）与钼酸盐在锑（III）和作为还原剂的抗坏血酸存在下反应，形成蓝色的砷钼蓝络合物。使用 Triton X - 114 将该络合物萃取到表面活性剂相中，并在 690 nm 处测量其吸光度。检测限、工作范围和相对标准偏差分别为 1 ng mL⁻¹、10 - 200 ng mL⁻¹ 和 1.2%。研究了常见离子的影响，并将该方法应用于测定环境、生物和商业采购化学品等各种样品中的痕量 As(III) 和 As(V)。
