Yim Hyeon Ju, Lim Jung Hwa, Kim Min Hee, Namgung Uk, Lee Sang Ryong, Jung In Chul
Department of Oriental Medicine, Daejeon University, Daejeon 300-716, Republic of Korea.
Evid Based Complement Alternat Med. 2012;2012:459894. doi: 10.1155/2012/459894. Epub 2012 May 15.
Background. Sibjeondaebo-tang (SJDBT) has been used to treat diverse disorders including neuropsychiatric disabilities in traditional Korean medicine. Objective. The present study aims to investigate the potential effects of SJDBT on neuroprotection against Aβ peptide-induced damage using in vitro culture and in vivo rat brain systems. Materials and Methods. PC12 cell viability was analyzed by MTT assay, and neurite arborizations and caspase 3 protein signals in cultured PC12 cells and in vivo cortical neurons were analyzed by immunofluorescence staining. Phospho-Erk1/2 protein was analyzed by immunofluorescence staining and western blot analysis. Results. In PC12 cells, atrophied cell body and reduced neurite extension by Aβ treatment were recovered by SJDBT treatment. Caspase 3 protein signals were increased in Aβ-treated PC12 cells, but SJDBT treatment decreased apoptotic cell death. Caspase 3 activation in cortical neurons, which was induced similarly by Aβ treatment, was reduced by SJDBT treatment. Furthermore, phospho-Erk1/2 protein levels, which had been decreased by Aβ treatment, were elevated in the cortical neurons by SJDBT treatment. Conclusion. These data show that SJDBT may play a role in protecting from damages induced by Aβ in neuronal tissue and further suggest that SJDBT can be explored as the potential therapeutic target for AD treatments in human.
背景。在传统韩医学中，四君子汤已被用于治疗包括神经精神障碍在内的多种疾病。目的。本研究旨在利用体外培养和体内大鼠脑系统，研究四君子汤对β淀粉样肽（Aβ）诱导损伤的神经保护潜在作用。材料与方法。通过MTT法分析PC12细胞活力，通过免疫荧光染色分析培养的PC12细胞和体内皮质神经元中的神经突分支和半胱天冬酶3蛋白信号。通过免疫荧光染色和蛋白质印迹分析磷酸化细胞外信号调节激酶1/2（p-Erk1/2）蛋白。结果。在PC12细胞中，四君子汤处理可恢复Aβ处理导致的萎缩细胞体和减少的神经突延伸。在Aβ处理的PC12细胞中半胱天冬酶3蛋白信号增加，但四君子汤处理可减少凋亡细胞死亡。四君子汤处理可降低Aβ处理同样诱导的皮质神经元中的半胱天冬酶3激活。此外，四君子汤处理可提高皮质神经元中因Aβ处理而降低的p-Erk1/2蛋白水平。结论。这些数据表明，四君子汤可能在保护神经元组织免受Aβ诱导的损伤中发挥作用，并进一步表明四君子汤可作为人类阿尔茨海默病治疗的潜在治疗靶点进行探索。
