School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China.
Ultrason Sonochem. 2013 Jan;20(1):125-32. doi: 10.1016/j.ultsonch.2012.05.006. Epub 2012 May 24.
In this study the potential use of an aqueous two phase system (ATPS) coupled with ultrasound for the extraction of lignans from Schisandra chinensis seeds was evaluated and optimized using response surface methodology (RSM). The main bioactive components, schizandrin (SA), schisantherin A (SAA) and deoxyschizandrin (DSA) were selected as markers. The partitioning behavior of lignans in different salt-types of ATPS was compared. The optimization ATPS of 25% (w/w) (NH(4))(2)SO(4) and 19% (w/w) ethanol were selected based on their higher upper phase partitioning coefficient (>74) and the recovery (>93%) for three markers. Using the optimized ATPS solvent, the RMS results showed 20:1 of solvent:solid, 800 W and 61.1 min were the optimal ultrasound assisted extraction conditions, under which 13.10mg/g SA, 1.87 mg/g SAA and 1.84 mg/g DSA were recovered in the upper phase, whereas the wasted stigmas accumulated in the lower phase. Compared with 80% ethanol (v/v) ultrasonic extraction, similar yields were obtained, but the present method exhibited higher extraction purity for the selective extraction of lignans from S. chinensis seeds.
在这项研究中，评估并优化了使用响应面法（RSM）通过双水相系统（ATPS）与超声联合从五味子种子中提取木脂素的潜在用途。选择主要生物活性成分五味子甲素（SA）、五味子丙素（SAA）和五味子醇甲（DSA）作为标记物。比较了木脂素在不同盐型 ATPS 中的分配行为。基于较高的上层分配系数（>74）和三种标记物的回收率（>93%），选择了 25%（w/w）（NH 4 ） 2 SO 4 和 19%（w/w）乙醇的优化 ATPS。使用优化的 ATPS 溶剂，RMS 结果表明，在最佳超声辅助提取条件下，溶剂：固体比为 20:1，功率为 800 W，时间为 61.1 min，在上层可回收 13.10mg/g SA、1.87mg/g SAA 和 1.84mg/g DSA，而浪费的柱头则积累在下层。与 80%乙醇（v/v）超声提取相比，该方法获得了相似的产率，但选择性提取五味子种子中的木脂素的提取纯度更高。
