从口腔黏膜和感染 HIV 儿童牙本质龋损中分离的白念珠菌的胶原酶活性差异。
Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children.
机构信息
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Mar;113(3):378-83. doi: 10.1016/j.oooo.2011.09.006.
OBJECTIVE
The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children.
STUDY DESIGN
To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120.
RESULTS
Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P < .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P > .05).
CONCLUSIONS
Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I collagen.
目的
本研究旨在比较分离自口腔黏膜（M）和有腔活性牙本质龋（CAD）的 HIV 感染儿童口腔白色念珠菌对 I 型胶原的降解作用。
设计
为了验证蛋白水解活性，将标本在脑心浸液培养基中培养，将上清液在 37°C 下孵育 12 小时，同时存在或不存在 I 型胶原蛋白，并使用 10％十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析。通过扫描仪对凝胶上的条带进行密度计分析，并使用柯达数字科学 EDAS 120 软件对图像进行分析。
结果
所有白色念珠菌的上清液均能降解 I 型胶原蛋白：M 来源的白色念珠菌平均降解 38.3％（SD 21.67），CAD 来源的白色念珠菌降解 54％（SD 25.94；Wilcoxon 检验：P<.05）。易患因素与 I 型胶原蛋白降解百分比之间无关联（Mann-Whitney 检验：P>.05）。
结论
HIV 感染儿童口腔不同部位的白色念珠菌具有 I 型胶原蛋白的蛋白水解活性。
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