de Carvalho Fabíola Galbiatti, Silva Danilo Souza, Hebling Josimeri, Spolidorio Luis Carlos, Spolidorio Denise Madalena Palomari
Department of Orthodontics and Pediatric Dentistry, Araraquara Dental School, São Paulo State University, UNESP, Brazil.
Arch Oral Biol. 2006 Nov;51(11):1024-8. doi: 10.1016/j.archoralbio.2006.06.001. Epub 2006 Aug 7.
This study determined the presence of mutans streptococci and Candida spp. in supragingival dental plaque and infected dentine of caries-free children, with early childhood caries and caries. Pooled samples of dental plaque and infected dentine were collected from 56 children aged 1-5 years, which were divided into 3 groups: early childhood caries (ECC); caries and caries-free. Infected dentine was collected in ECC and caries groups to compare the frequency of these microorganisms in the collected sites. The samples were inoculated in SB20 and SA medium, for mutans streptococci and Candida spp., respectively, and incubated at 37 degrees C for 48 h. Colony growth was verified and the identification was performed by biochemical tests and CHROMagar Candida. Fisher's test or chi-square (chi(2)) were applied (p=0.05). The more prevalent species were S. mutans and Candida albicans in ECC (85.4% and 60.4%, respectively), independently of the sample site. S. mutans only was significantly associated with carious teeth, whether in early childhood caries or not. However, the frequency of C. albicans in ECC was higher when compared to caries and caries-free groups. There is a significant association between the presence of C. albicans and early childhood caries.
本研究确定了无龋儿童、患有幼儿龋和龋齿的儿童龈上牙菌斑及感染牙本质中变形链球菌和念珠菌属的存在情况。从56名1至5岁儿童中收集牙菌斑和感染牙本质的混合样本，这些儿童被分为3组：幼儿龋（ECC）组、龋齿组和无龋组。在ECC组和龋齿组中收集感染牙本质，以比较这些微生物在所收集部位的出现频率。样本分别接种于用于培养变形链球菌的SB20培养基和用于培养念珠菌属的SA培养基中，并在37℃下孵育48小时。通过生化试验和CHROMagar念珠菌培养基验证菌落生长并进行鉴定。应用Fisher检验或卡方检验（χ²检验）（p = 0.05）。在ECC组中，无论样本部位如何，最常见的菌种是变形链球菌和白色念珠菌（分别为85.4%和60.4%）。无论是否患有幼儿龋，仅变形链球菌与龋坏牙齿显著相关。然而，与龋齿组和无龋组相比，ECC组中白色念珠菌的出现频率更高。白色念珠菌与幼儿龋之间存在显著关联。
