健康学龄前和学龄儿童口腔白色念珠菌携带情况

Oral Candida albicans carriage in healthy preschool and school children.

作者信息

Rozkiewicz D, Daniluk T, Zaremba M L, Cylwik-Rokicka D, Stokowska W, Pawińska M, Dabrowska E, Marczuk-Kolada G, Waszkiel D

机构信息

Department of Pediatric Infectious Diseases, Medical University of Białystok, Poland.

出版信息

Adv Med Sci. 2006;51 Suppl 1:187-90.

PMID:17458089

Abstract

PURPOSE

The purpose of the present study was to detect Candida albicans carriage in the oral cavity of healthy preschool and school children. The second aim was the determination of correlation between C. albicans occurrence and dental caries in children population.

MATERIAL AND METHODS

The samples for mycological examinations were collected from the pharynx and supragingival plaque, and carious lesions in 102 children, aged 4-7 years (preschool children) and 104 children and adolescents, aged 12 and 18 (school children). All samples were cultured directly on Sabouraud agar medium. Isolated yeasts were identified based on API 20C AUX (bioMérieux).

RESULTS

A total of 123 C. albicans strains were isolated, in which 61 (49.6%) derived from supragingival plaque, 48 (39%)--from carious lesions, and 14 (11.4%)--from pharyngeal swabs. C. albicans was isolated from the samples of single material in 61 children (35--school children, 26 --preschool children) while from the rest of 29 children, C. albicans was isolated from two (25x) or three materials (4x). C. albicans was detected in 48/75 (64%) children with dental caries; the rate was statistically significantly higher as compared to the overall number of children with C. albicans carriage (90/206; 43.7%) (p = 0.0026). Similar results was obtained in preschool children (38/61; 62.3% and 47/102; 46.1%, respectively) (p = 0.0449), as in school children (10/14; 71.4% and 43/104; 41.3%, respectively) (p = 0.0336).

CONCLUSIONS

Candida albicans was observed in the oral cavity of healthy children with high (approximately 40%)--comparable rate in school and preschool children (p > 0.05). 2) C. albicans was isolated with high comparable rate from carious lesions in preschool and school children. The statistically significant differences between the rate of C. albicans in carious lesions in preschool children (62.3%) and school children (71.4%) and the overall number of children with C. albicans carriage in the oral cavity of children in both age groups (p < 0.05) were showed.

摘要

目的

本研究旨在检测健康学龄前儿童和学龄儿童口腔中的白色念珠菌携带情况。第二个目的是确定儿童群体中白色念珠菌的出现与龋齿之间的相关性。

材料与方法

从102名4至7岁的儿童（学龄前儿童）以及104名12至18岁的儿童和青少年（学龄儿童）的咽部、龈上菌斑和龋损处采集用于真菌学检查的样本。所有样本直接接种于沙氏琼脂培养基上。基于API 20C AUX（生物梅里埃公司）对分离出的酵母菌进行鉴定。

结果

共分离出123株白色念珠菌菌株，其中61株（49.6%）来自龈上菌斑，48株（39%）来自龋损处，14株（11.4%）来自咽拭子。在61名儿童（35名学龄儿童，26名学龄前儿童）的单一材料样本中分离出白色念珠菌，而在其余29名儿童中，白色念珠菌从两种（25例）或三种材料（4例）中分离得到。在48/75（64%）患有龋齿的儿童中检测到白色念珠菌；与白色念珠菌携带儿童的总数（90/206；43.7%）相比，该比例在统计学上显著更高（p = 0.0026）。学龄前儿童（分别为38/61；62.3%和47/102；46.1%）（p = 0.0449）以及学龄儿童（分别为10/14；71.4%和43/104；41.3%）（p = 0.0336）也得到了类似结果。

结论

1）在健康儿童口腔中观察到白色念珠菌，其携带率较高（约40%），在学龄儿童和学龄前儿童中相当（p > 0.05）。2）在学龄前儿童和学龄儿童的龋损处分离出白色念珠菌的比例相当高。学龄前儿童（62.3%）和学龄儿童（71.4%）龋损处白色念珠菌分离率与两个年龄组儿童口腔中白色念珠菌携带儿童总数之间存在统计学显著差异（p < 0.05）。