Veneziani Bianca Maria, De Placido Sabino
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università degli Studi di Napoli Federico II, via Pansini 5, 80131, Naples, Italy.
Cell Tissue Bank. 2013 Jun;14(2):153-8. doi: 10.1007/s10561-012-9320-6. Epub 2012 Jun 6.
Identification of breast cancer stem cells, within the context of the tumor tissue, requires an efficient and standardized method to preserve the functional features of living cells. Although isolating cancer stem cells can be laborious and time-consuming, minimal processing may be advantageous for the banking of specimens from which cultures are not immediately needed. Homogenization of banking procedures will result in a reliable network of biorepositories for cooperative studies and several research groups are focusing on the issues of tissue banks for translational medicine. Most tissue banks collect and freeze unprocessed cancer specimens, which cannot therefore be used to generate viable cells. We discuss the principal issues of biobanking breast cancer living cells and protocols for mammospheres formation from single cell suspension of tumor cells.
在肿瘤组织背景下鉴定乳腺癌干细胞，需要一种高效且标准化的方法来保留活细胞的功能特性。尽管分离癌症干细胞可能既费力又耗时，但对于那些并非立即需要用于培养的标本库而言，尽量减少处理步骤可能具有优势。标本库操作流程的标准化将形成一个可靠的生物样本库网络，以开展合作研究，并且几个研究小组正专注于转化医学组织库的相关问题。大多数组织库收集并冷冻未经处理的癌症标本，因此这些标本无法用于生成活细胞。我们讨论了生物样本库保存乳腺癌活细胞的主要问题以及从肿瘤细胞单细胞悬液形成乳腺球的方案。
