Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, AB, Canada.
Arnie Charbonneau Cancer Institute, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2022;2508:45-58. doi: 10.1007/978-1-0716-2376-3_5.
Biorepositories of fresh frozen and formalin-fixed paraffin-embedded tissues have been foundational to many molecular cancer research studies. Collections of these materials, however, do not enable the establishment of short-term cultures, cell lines, or patient-derived xenograft models for functional studies. Also, intact dissociated cells that are required for some single-cell analyses cannot be obtained from these material types. Adding viable tumor banking to the repertoire of routine cancer biobanking would increase the value of samples collected. This chapter outlines procedures for processing and storing blood and tissue specimens viably in order to expand the future utility of the samples collected. We provide practical tips that can be used by banks and other researchers seeking to incorporate the cryopreservation of viable materials as part of their overall biobanking strategies.
生物库中的新鲜冷冻和福尔马林固定石蜡包埋组织一直是许多分子癌症研究的基础。然而，这些材料的收集并不能建立短期培养物、细胞系或患者来源的异种移植模型进行功能研究。此外，一些单细胞分析所需的完整分离细胞也无法从这些材料类型中获得。将有活力的肿瘤储存添加到常规癌症生物库的范围内，将增加收集样本的价值。本章概述了以有活力的方式处理和储存血液和组织标本的过程，以便扩大未来对收集样本的利用。我们提供了实际的技巧，可供银行和其他研究人员使用，以将有活力的材料的冷冻保存纳入其整体生物库策略的一部分。
