Balabanov V P, Pustovoĭt K S, Zavil'gel'skiĭ G B
Mol Biol (Mosk). 2012 Mar-Apr;46(2):269-75.
Antirestriction proteins ArdA and ArdB are specific inhibitors of the type I restriction-modification enzymes. The transmissible plasmid R64 ardA and yfeB (ardB) genes were cloned in pUC18 and pZE21 vectors. It was shown that the R64 ArdA and ArdB proteins inhibit only restriction activity of the type I restriction-modification enzyme (EcoKI) in Escherichia coli K12 cells. The dependence of the effectiveness of the antirestriction activity of the ArdA and ArdB proteins on the intracellular concentration was determined. Antirestriction activity of ArdB is independent from the ClpXP protease. Transcription of yfeB (ardB) gene in R64 plasmid is realized from the yfeA promoter.
抗限制蛋白ArdA和ArdB是I型限制修饰酶的特异性抑制剂。可转移质粒R64的ardA和yfeB（ardB）基因被克隆到pUC18和pZE21载体中。结果表明，R64的ArdA和ArdB蛋白仅抑制大肠杆菌K12细胞中I型限制修饰酶（EcoKI）的限制活性。测定了ArdA和ArdB蛋白抗限制活性的有效性与细胞内浓度的关系。ArdB的抗限制活性不依赖于ClpXP蛋白酶。R64质粒中yfeB（ardB）基因的转录是从yfeA启动子开始的。
