HIV-1 Nef 蛋白与 40S 小核糖体亚基的两个成分相互作用，即 RPS10 蛋白和 18S rRNA。
The HIV-1 Nef protein interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA.
机构信息
Department of Virology, University of Franche-Comte, UPRES EA 4266, IFR 133 INSERM, CHU Besancon, F-25030, Besançon, France.
出版信息
Virol J. 2012 Jul 10;9:103. doi: 10.1186/1743-422X-9-103.
BACKGROUND
Human immunodeficiency virus type 1 (HIV-1) Nef-encoded protein plays key functions at almost all stages of the viral life cycle, but its role in translation is largely unknown.
METHODS
To determine the effect of Nef on translation we used an in vitro translation assay. The detection of Nef/RPS10 complexes and the presence of 18S rRNA and tRNAs in the complexes were performed by coimmunoprecipitation and RT-PCR assay.
RESULTS
We observed that the HIV-1 Nef protein specifically impaired translation in vitro. We observed the interaction of Nef with RPS10 by coimmunoprecipitation assay. In addition 18S rRNA and tRNAs were present in the Nef/RPS10 complexes.
CONCLUSIONS
Our results are consistent with a model in which the Nef protein by binding to two components of the 40S small ribosomal subunit, RPS10 and 18S rRNA, and to a lesser extent to tRNAs, could lead to decreased protein synthesis.
背景
人类免疫缺陷病毒 1 型（HIV-1）Nef 编码的蛋白在病毒生命周期的几乎所有阶段都发挥着关键作用，但它在翻译中的作用在很大程度上是未知的。
方法
为了确定 Nef 对翻译的影响，我们使用了体外翻译测定法。通过共免疫沉淀和 RT-PCR 检测来检测 Nef/RPS10 复合物的存在以及复合物中 18S rRNA 和 tRNA 的存在。
结果
我们观察到 HIV-1 Nef 蛋白特异性地损害了体外翻译。我们通过共免疫沉淀检测观察到 Nef 与 RPS10 的相互作用。此外，18S rRNA 和 tRNA 存在于 Nef/RPS10 复合物中。
结论
我们的结果与这样一种模型一致，即 Nef 蛋白通过与 40S 小核糖体亚基的两个成分（RPS10 和 18S rRNA）结合，并在较小程度上与 tRNA 结合，可能导致蛋白质合成减少。
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