Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
Dermatol Surg. 2012 Aug;38(8):1284-93. doi: 10.1111/j.1524-4725.2012.02454.x. Epub 2012 Jun 5.
Topical treatment with vitamin C has been used to treat photoaged skin and as a skin whitener, but no standard procedure exists for percutaneous delivery.
To compare skin histology and the permeation of ascorbic acid 2-glucoside (AA2G) after fractional and conventional carbon dioxide (CO(2) ) laser pretreatment.
The effect on porcine skin of treatment with different strengths of fractional and conventional CO(2) laser treatment was examined using scanning electron microscopy and transmission electron microscopy. Permeation of AA2G through porcine skin was tested in vitro using a Franz diffusion chamber. In vivo changes in fluorescein thiocyanate permeability in nude mice were examined using confocal laser scanning microscopy.
Fractional CO(2) laser treatment with four or fewer passes caused less disruption than conventional laser treatment at the same fluence. AA2G permeation using four passes of fractional laser treatment was similar to that seen with conventional CO(2) laser treatment of the same fluence. Changes in permeability and in depth of permeation were higher with conventional than fractional laser treatment.
Fractional CO(2) laser treatment can cause similar transdermal delivery of AA2G to conventional laser treatment with less skin disruption and a different pattern of histologic change.
局部使用维生素 C 已被用于治疗光老化皮肤和美白皮肤，但不存在经皮给药的标准程序。
比较经皮给药后，分幅与常规二氧化碳（CO2）激光预处理对皮肤组织学和抗坏血酸 2-葡萄糖苷（AA2G）渗透的影响。
采用扫描电子显微镜和透射电子显微镜观察不同强度分幅和常规 CO2 激光治疗对猪皮的影响。采用 Franz 扩散室在体测试 AA2G 通过猪皮的渗透情况。使用共聚焦激光扫描显微镜观察在体条件下，异硫氰酸荧光素通透性的变化。
与相同能量密度的常规激光治疗相比，能量密度较低的 4 次以下分幅激光治疗对皮肤的破坏较小。分幅激光治疗 4 次的 AA2G 渗透与相同能量密度的常规 CO2 激光治疗相似。与分幅激光治疗相比，常规激光治疗引起的渗透性和渗透深度的变化更大。
与常规激光治疗相比，分幅 CO2 激光治疗可导致类似的 AA2G 经皮给药，对皮肤的破坏较小，组织学改变模式不同。
