Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University, Kolhapur 416 004, Maharashtra, India.
J Photochem Photobiol B. 2012 Aug 1;113:63-9. doi: 10.1016/j.jphotobiol.2012.05.006. Epub 2012 May 18.
Current study employs fluorescence spectroscopy, UV-Vis absorbance spectroscopy, dynamic light scattering (DLS) and cyclic voltammetry (CV) to investigate the interaction of safranine dye with spherical shaped silver nanoparticles (AgNPs) coated with trisodium citrate. In fluorescence spectroscopic study we used the AgNPs and safranine dye as component molecules for the construction of FRET, whereas AgNPs serve as donor fluorophore and safranine as acceptor. The fluorescence quenching of AgNPs followed by sensitization of safranine occurs almost simultaneously by addition of safranine dye with different concentrations, indicating fluorescence energy transfer observed between them. Interaction between safranine and AgNPs is also confirmed by using UV-Vis absorption spectroscopy. Addition of safranine results in the significant decrease in the absorbance of AgNPs at 423 nm and simultaneous increase in the absorbance of safranine at 518, 276 and 248 nm which is indication of rapid binding of safranine molecules with AgNPs. However CV measurements reveals that the safranine molecule does not alter the redox properties of the AgNPs but the safranine molecule lose their redox properties upon getting bonded with AgNPs. This clearly confirms that the safranine molecules get attached on the surface of AgNPs which was also supported by the DLS as well as zeta potential measurement.
本研究采用荧光光谱法、紫外可见吸收光谱法、动态光散射（DLS）和循环伏安法（CV）研究了与柠檬酸三钠包覆的球形银纳米粒子（AgNPs）相互作用的藏红染料。在荧光光谱研究中，我们使用 AgNPs 和藏红染料作为构建 FRET 的组成分子，而 AgNPs 作为供体荧光团，藏红作为受体。随着不同浓度的藏红染料的加入，AgNPs 的荧光猝灭伴随着藏红的敏化几乎同时发生，表明它们之间观察到荧光能量转移。通过使用紫外可见吸收光谱也证实了藏红与 AgNPs 之间的相互作用。藏红的加入导致 AgNPs 在 423nm 处的吸收显著降低，同时藏红在 518、276 和 248nm 处的吸收增加，这表明藏红分子与 AgNPs 快速结合。然而，CV 测量表明，藏红分子不会改变 AgNPs 的氧化还原性质，但藏红分子在与 AgNPs 结合后失去其氧化还原性质。这清楚地证实了藏红分子附着在 AgNPs 的表面上，这也得到了 DLS 以及zeta 电位测量的支持。
