Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products (LATVPEP), Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia.
Res Vet Sci. 2012 Dec;93(3):1151-9. doi: 10.1016/j.rvsc.2012.05.005. Epub 2012 Jun 5.
The probiotic properties of Bacillus strains isolated from Artemia culture and the effect of marine stress on viability and survival were investigated, as well as the changes occurring in their properties. Analyses showed that these bacteria corresponded to the genus Bacillus sp. Antagonism and adherence assays revealed that Bacillus strains have an inhibitory effect against tested pathogenic bacteria and are fairly adherent. Normal and starved cells showed different enzymatic profiles. Challenge tests performed with Artemia larvae provided evidence that the tested Bacillus strains were neither pathogenic nor toxic to the host and conferred protection for Artemia culture against pathogens. The tested strains maintained their viability and their probiotic properties during the period of study. The results suggest that the tested strains have suffered changes allowing them to survive in seawater in the absence of nutrients and outside their natural host, identifying them as potential probiotic candidates for Artemia culture.
研究了从丰年虫卵培养物中分离出的芽孢杆菌的益生菌特性以及海洋应激对其生存能力和生存能力的影响，以及它们特性发生的变化。分析表明，这些细菌属于芽孢杆菌属。拮抗和粘附试验表明，芽孢杆菌对测试的致病菌具有抑制作用，并且具有较强的粘附性。正常和饥饿细胞显示出不同的酶谱。用丰年虫卵幼虫进行的挑战试验提供了证据，表明测试的芽孢杆菌菌株既对宿主无致病性也无毒性，并且可以保护丰年虫卵培养物免受病原体的侵害。在研究期间，测试菌株保持了其生存能力和益生菌特性。结果表明，测试菌株发生了变化，使它们能够在缺乏营养物质的海水中以及在其自然宿主之外生存，将它们鉴定为丰年虫卵培养物的潜在益生菌候选物。
