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利用混合设计构建由椰枣(Phoenix dactylifera L.)果提取物和潜在益生菌芽孢杆菌组成的联合体,以防止卤虫培养物中的弧菌病。

Use of mixture design to construct a consortium of date palm (Phoenix dactylifera L.) fruit extract and potentially probiotic Bacillus strain to confer protection against vibriosis in Artemia culture.

机构信息

Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products (LATVPEP), Faculty of Pharmacy, University of Monastir, Monastir, 5000, Tunisia.

出版信息

J Sci Food Agric. 2013 Dec;93(15):3850-5. doi: 10.1002/jsfa.6323. Epub 2013 Aug 19.

DOI:10.1002/jsfa.6323
PMID:23893402
Abstract

BACKGROUND

To find a biological control treatment applicable to shrimp hatcheries, the influence of the dietary administration of a consortium of two date palm (Phoenix dactylifera L.) fruit extracts and potentially probiotic Bacillus strain on Artemia culture was studied. Challenge tests were performed with virulent Vibrio alginolyticus to assess the protective effects of Deglet Nour and Degla fruit extracts alone or in conjunction with potentially probiotic Bacillus strain.

RESULTS

Under the conducted bioassays, where Deglet Nour and Degla fruit extracts were used singly or in association with potentially probiotic Bacillus strain, the survival and growth rates of Artemia nauplii were improved. Nauplii protection against virulent Vibrio strain was highest using a feed comprising 2.7% Deglet Nour/97.3% Deglat extract and no Bacillus bacteria.

CONCLUSION

The present findings provide evidence that the use of palm fruit extracts singly or in conjunction with potentially probiotic Bacillus strain has tremendous potential as a biocontrol treatment for Artemia culture, particularly with regard to improving culture conditions and the ability to inhibit bacterial pathogens under in vivo conditions.

摘要

背景

为了寻找适用于虾孵化场的生物防治处理方法,研究了日粮中添加两种椰枣(Phoenix dactylifera L.)果提取物和潜在益生菌芽孢杆菌对卤虫培养的影响。用致病性溶藻弧菌进行了挑战试验,以评估 Deglet Nour 和 Degla 果提取物单独或与潜在益生菌芽孢杆菌联合使用的保护作用。

结果

在进行的生物测定中,单独使用 Deglet Nour 和 Degla 果提取物或与潜在益生菌芽孢杆菌联合使用时,卤虫无节幼体的存活率和生长率都有所提高。使用包含 2.7% Deglet Nour/97.3% Deglat 提取物和无芽孢杆菌的饲料对致病性弧菌菌株的保护作用最高。

结论

本研究结果表明,单独使用或与潜在益生菌芽孢杆菌联合使用椰枣果提取物具有作为卤虫培养物生物防治处理方法的巨大潜力,特别是在改善培养条件和抑制体内条件下细菌病原体的能力方面。

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