Kim Hyo-Jin, Tark Dong-Seob, Lee Yoon-Hee, Kim Min-Jeong, Lee Won-Yong, Cho In-Soo, Sohn Hyun-Joo, Yokoyama Takashi
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi-do, Korea.
J Vet Med Sci. 2012 Oct;74(10):1377-80. doi: 10.1292/jvms.12-0061. Epub 2012 Jun 5.
Elk prion protein (PrP(C)) has been confirmed to be capable of rendering rabbit epithelial RK13 cells permissive to temporal infection by chronic wasting disease (CWD) prions. The present study satisfactorily generated persistently CWD prion-affected RK13 cells (RKC1-11) using elk PrP(C) expressing cells (elkRK13) that were generated via the lentiviral expression system with high efficiency. The elkRK13 cells have been shown to be permissive to accumulation of abnormal isoforms of prion protein (PrP(Sc)) resulting from CWD prions up to 97 serial passages thus far. This novel prion-affected cell line will help facilitate investigation of the molecular basis of CWD prion pathogenesis and confirmation of CWD prion infectivity in vitro.
麋鹿朊病毒蛋白（PrP(C)）已被证实能够使兔上皮RK13细胞易于受到慢性消耗病（CWD）朊病毒的暂时感染。本研究通过高效的慢病毒表达系统成功地利用表达麋鹿PrP(C)的细胞（elkRK13）产生了持续受CWD朊病毒影响的RK13细胞（RKC1-11）。迄今为止，elkRK13细胞已被证明易于积累由CWD朊病毒产生的朊病毒蛋白异常异构体（PrP(Sc)），传代多达97次。这种新型的受朊病毒影响的细胞系将有助于促进对CWD朊病毒发病机制分子基础的研究以及体外CWD朊病毒感染性的确认。
