Tareq K M A, Akter Quzi Sharmin, Khandoker M A M Yahia, Tsujii Hirotada
Laboratory of Animal Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan.
J Reprod Dev. 2012;58(6):621-8. doi: 10.1262/jrd.2012-064. Epub 2012 Jun 5.
Selenium (Se) and vitamin E (Vit-E), as integral parts of antioxidant systems, play important roles for sperm and embryos in vitro. In this study, the effects of Se and Vit-E on the maturation, in vitro fertilization and culture to blastocysts of porcine oocytes and accumulation of ammonia in the culture medium during different development stages were investigated. The maturation was performed in modified tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, the fertilization medium was modified Tyrode's albumin lactate pyruvate (mTALP), and the embryo culture medium was modified North Carolina State University (mNCSU)-23. Se in the form of sodium selenite (SS) and seleon-L-methionine (SeMet) and Vit-E at different concentrations were also used. The incorporation and oxidation of (14)C(U)-glucose were assessed with a liquid scintillation counter. In this study, SeMet and SeMet+Vit-E increased oocyte maturation, fertilization and incorporation and oxidation of (14)C(U)-glucose significantly (P<0.05) compared with the control and other treatments. In addition, embryo development, specifically in terms of the numbers of morulae and blastocysts, significantly increased (P<0.05) with SeMet and SeMet+Vit-E. In contrast, the accumulation of ammonia was reduced with SeMet and SeMet+Vit-E compared with other treatments. These findings indicate that SeMet and SeMet+Vit-E may play important roles in reducing the accumulation of ammonia and subsequently in increasing the rate of maturation of porcine oocytes and fertilization, as well as development of the blastocyst and utilization of glucose in in vitro maturation, fertilization and culture to blastocysts of porcine oocytes.
硒（Se）和维生素E（Vit-E）作为抗氧化系统的组成部分，在体外对精子和胚胎起着重要作用。本研究探讨了Se和Vit-E对猪卵母细胞成熟、体外受精及囊胚培养的影响，以及不同发育阶段培养基中氨的积累情况。成熟培养在添加10%（v/v）猪卵泡液的改良组织培养液（mTCM）-199中进行，受精培养液为改良的泰勒白蛋白乳酸丙酮酸溶液（mTALP），胚胎培养液为改良的北卡罗来纳州立大学培养液（mNCSU）-23。还使用了不同浓度的亚硒酸钠（SS）形式的Se、硒-L-蛋氨酸（SeMet）和Vit-E。用液体闪烁计数器评估（14）C（U）-葡萄糖的掺入和氧化情况。在本研究中，与对照组和其他处理相比，SeMet和SeMet+Vit-E显著提高了卵母细胞成熟率、受精率以及（14）C（U）-葡萄糖的掺入和氧化（P<0.05）。此外，SeMet和SeMet+Vit-E显著增加了胚胎发育，特别是桑椹胚和囊胚的数量（P<0.05）。相比之下，与其他处理相比，SeMet和SeMet+Vit-E降低了氨的积累。这些发现表明，SeMet和SeMet+Vit-E在减少氨的积累、进而提高猪卵母细胞体外成熟、受精率以及囊胚发育和葡萄糖利用方面可能发挥重要作用。
