Tana Chonthicha, Somsak Pareeya, Piromlertamorn Waraporn, Sanmee Usanee
Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
CMEx Fertility Center, Center of Medical Excellence, Chiang Mai University, Chiang Mai, Thailand.
Clin Exp Reprod Med. 2022 Mar;49(1):26-32. doi: 10.5653/cerm.2021.04770. Epub 2022 Feb 28.
We investigated the effect of supplementing fertilization medium and/or culture medium with astaxanthin (AST) on the two phases of in vitro fertilization: gamete fertilization and embryo development.
Mouse cumulus-oocyte complexes were divided into four groups with 5 µM AST added to the fertilization medium (group 3, n=300), culture medium (group 2, n=300), or both media (group 4, n=290). No AST was added to the control group (group 1, n=300).
The fertilization rate was significantly higher (p<0.001) in the groups using AST supplemented fertilization medium (group 3, 79.0%; group 4, 81.4%) than those without AST (group 1, 56.3%; group 2, 52.3%). The blastocyst rate calculated from the two-cell stage was significantly lower (p<0.001) in the groups using AST-supplemented embryo culture medium (group 2, 58.0%; group 4, 62.3%) than in those without AST (group 1, 82.8%; group 3, 79.8%). The blastocyst rate calculated from the number of inseminated oocytes was highest in group 3 (189/300, 63.0%) and lowest in group 2 (91/300, 30.3%) with statistical significance compared to other groups (p<0.001). There were significantly higher numbers of cells in the inner cell mass and trophectoderm, as well as significantly higher total blastocyst cell counts, in group 3 than in the control group.
An increased blastocyst formation rate and high-quality blastocysts were found only in the fertilization medium that had been supplemented with AST. In contrast, AST supplementation of the embryo culture medium was found to impair embryo development.
我们研究了在受精培养基和/或培养基中添加虾青素（AST）对体外受精两个阶段的影响：配子受精和胚胎发育。
将小鼠卵丘-卵母细胞复合体分为四组，分别在受精培养基中添加5μM AST（第3组，n = 300）、培养基中添加5μM AST（第2组，n = 300）或两种培养基中均添加5μM AST（第4组，n = 290）。对照组（第1组，n = 300）不添加AST。
使用添加AST的受精培养基的组（第3组，79.0%；第4组，81.4%）的受精率显著高于未添加AST的组（第1组，56.3%；第2组，52.3%）（p<0.001）。从二细胞阶段计算的囊胚率在使用添加AST的胚胎培养基的组（第2组，58.0%；第4组，62.3%）中显著低于未添加AST的组（第1组，82.8%；第3组，79.8%）（p<0.001）。根据受精卵母细胞数量计算的囊胚率在第3组中最高（189/300，63.0%），在第2组中最低（91/300，30.3%），与其他组相比具有统计学意义（p<0.001）。第3组的内细胞团和滋养外胚层中的细胞数量显著多于对照组，囊胚细胞总数也显著更高。
仅在添加了AST的受精培养基中发现囊胚形成率增加和高质量囊胚。相反，在胚胎培养基中添加AST会损害胚胎发育。
