Devaux C, Boucraut J, Poirier G, Corbeau P, Rey F, Benkirane M, Perarnau B, Kourilsky F, Chermann J C
INSERM-U322, Unité de Recherches sur les Rétrovirus et Maladies Associées, Marseille, France.
Res Immunol. 1990 May-Jun;141(4-5):357-72. doi: 10.1016/0923-2494(90)90026-u.
We attempted to select monoclonal antibodies (mAb) which reacted with T-cell surface molecules and were able to interfere with the human immunodeficiency virus type 1 (HIV1) replicative cycle in the MT4 T-leukaemic cell line. In comparison with OKT4A, an anti-CD4 mAb, only one mAb, HC11.151.1, was found to significantly delay HIV-induced cytopathic effect on MT4 cells among the 15 mAb tested which reacted with MT4 cell surface antigens. Biochemical and immunological characterization of HC11.151.1 demonstrated its specificity for beta 2-microglobulin (beta 2m), the light chain of human leukocyte antigen (HLA) class I molecules. Other beta 2m-specific mAb were tested in order to assess whether this effect represented an intrinsic capacity of HC11.151.1 or whether it was a common feature shared by all anti-beta 2m mAb. Three (B1.1G6, B2.62.2 and BBM1) of the four anti-beta 2m mAb demonstrated the same protective effect, whereas C21.48A, which was devoid of a functional effect, was directed towards a beta 2m epitope involved in binding to the HLA class I heavy chain molecule. The physiological relevance of this observation is discussed.
我们试图筛选出能与T细胞表面分子发生反应，并能在MT4 T白血病细胞系中干扰1型人类免疫缺陷病毒（HIV-1）复制周期的单克隆抗体（mAb）。与抗CD4单克隆抗体OKT4A相比，在所检测的15种与MT4细胞表面抗原发生反应的单克隆抗体中，仅有一种单克隆抗体HC11.151.1能显著延缓HIV对MT4细胞的致细胞病变效应。对HC11.151.1进行的生化和免疫学特性分析表明，它对人白细胞抗原（HLA）I类分子的轻链β2-微球蛋白（β2m）具有特异性。我们检测了其他β2m特异性单克隆抗体，以评估这种效应是HC11.151.1的固有特性，还是所有抗β2m单克隆抗体共有的特征。四种抗β2m单克隆抗体中的三种（B1.1G6、B2.62.2和BBM1）表现出相同的保护作用，而无功能效应的C21.48A则针对与HLA I类重链分子结合的β2m表位。本文讨论了这一观察结果的生理相关性。
