Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, People's Republic of China.
Curr Microbiol. 2012 Sep;65(3):313-8. doi: 10.1007/s00284-012-0157-9. Epub 2012 Jun 7.
Tuberculous pleurisy is one of the common extrapulmonary tuberculosis diseases. However, the diagnosis of tuberculous pleurisy still lacks a useful and effective tool, mainly due to paucity of Mycobacterium tuberculosis organisms in pleural effusion. Previous studies have confirmed that the MPT64 protein is highly specific and is secreted only by M. tuberculosis (MTB) complex. Therefore, in this study, we developed ELISA based on recombinantly expressed MPT64 in combination with rabbit polyclonal antibodies. The ELISA-MPT64 method was validated using MTB strains and tested against clinical samples. Nested PCR, Löwenstein-Jensen (L-J) culture and smear microscopy were employed as the comparative tools for assessing the performance of the assay. Our results demonstrate that the newly established ELISA-MPT64 technique is a rapid and useful tool for the diagnosis of tuberculous pleurisy.
结核性胸膜炎是常见的肺外结核病之一。然而，结核性胸膜炎的诊断仍然缺乏一种有用和有效的工具，主要是由于胸腔积液中缺乏结核分枝杆菌。先前的研究已经证实，MPT64 蛋白具有高度特异性，仅由结核分枝杆菌（MTB）复合体分泌。因此，在这项研究中，我们开发了一种基于重组表达的 MPT64 的 ELISA，并结合兔多克隆抗体。使用 MTB 菌株对 ELISA-MPT64 方法进行了验证，并对临床样本进行了测试。巢式 PCR、Löwenstein-Jensen（L-J）培养和涂片显微镜检查被用作评估该检测方法性能的比较工具。我们的结果表明，新建立的 ELISA-MPT64 技术是一种快速而有用的结核性胸膜炎诊断工具。
