Etablissement Français du Sang Bourgogne Franche-Comté, Laboratoire d'hématologie-immunologie-biologie moléculaire, Besançon F-25020, France.
Cytometry A. 2012 Aug;81(8):718-24. doi: 10.1002/cyto.a.22072. Epub 2012 Jun 6.
Diagnosis of blastic plasmacytoid dendritic cell neoplasm (BPDCN) or plasmacytoid dendritic cell leukemia (pDCL) is mainly based on immunophenotypical characterization of leukemic cells in blood or bone marrow samples. We tested by flow cytometry intracellular expression of the proto-oncogene T-cell leukemia 1 (TCL1), as well as membrane and intracellular expression of immunoglobulin-like transcript 7 (ILT7) in 21 pDCL samples and 61 non-pDC acute leukemia samples [i.e., 14 B-acute lymphoblastic leukemia (B-ALL), 9 T-ALL and 38 acute myeloid leukemia (AML)]. TCL1 is highly expressed in all pDCL samples while at a statistically lower level in all B-ALL and 34% of AML. Statistical analysis shows that intensity of TCL1 expression is a good marker for differential diagnosis of pDCL versus other acute leukemia (area under the receiver-operating characteristic curve, [AUC]: 0.96). By contrast, ILT7 positivity is limited to few pDCL samples and cannot be useful for diagnosis purpose. In conclusion, high intracellular intensity of TCL1 expression is currently the best marker for pDC lineage assignment by flow cytometry, which is particularly useful to distinguish pDCL from CD4(+) CD56(+/-) undifferentiated or monoblastic acute leukemia. Thus, intracellular TCL1 detection should be included in acute leukemia diagnosis panels used in hematology laboratories. © 2012 International Society for Advancement of Cytometry.
诊断母细胞性浆细胞样树突细胞肿瘤（BPDCN）或浆细胞样树突细胞白血病（pDCL）主要基于血液或骨髓样本中白血病细胞的免疫表型特征。我们通过流式细胞术检测了 21 例 pDCL 样本和 61 例非 pDC 急性白血病样本[即 14 例 B-急性淋巴细胞白血病（B-ALL）、9 例 T-ALL 和 38 例急性髓系白血病（AML）]中原癌基因 T 细胞白血病 1（TCL1）的细胞内表达，以及免疫球蛋白样转录物 7（ILT7）的膜和细胞内表达。TCL1 在所有 pDCL 样本中均高度表达，而在所有 B-ALL 和 34%的 AML 中表达水平较低。统计分析表明，TCL1 表达强度是区分 pDCL 与其他急性白血病的良好标志物（受试者工作特征曲线下面积，[AUC]：0.96）。相比之下，ILT7 阳性仅限于少数 pDCL 样本，不能用于诊断目的。总之，细胞内 TCL1 表达强度高是目前流式细胞术用于 pDC 谱系鉴定的最佳标志物，特别有助于将 pDCL 与 CD4(+) CD56(+/-)未分化或单核细胞性急性白血病区分开来。因此，细胞内 TCL1 检测应包含在血液学实验室使用的急性白血病诊断面板中。
