Stehr C M, Myers M S
Environmental Conservation Division, National Oceanic and Atmospheric Administration, Seattle, Washington 98112.
Toxicol Pathol. 1990;18(3):362-72. doi: 10.1177/019262339001800302.
The ultrastructure and histology of cholangiocellular carcinomas from feral English sole (Parophyrs vetulus) living in polluted waterways of Puget Sound, WA. are described. Electron microscopy confirmed that biliary epithelial cells were the main proliferative cell type composing this variety of neoplasm. The arrangement of these cells varied from well-organized multiple bile duct-like structures to disorganized multilayered sheets of poorly differentiated biliary epithelial cells. A fibrous stroma consisting of multiple layers of collagen fibers and fibroblasts, with macrophages and various blood cell types scattered among these layers occurred between bile duct-like structures or aggregates of biliary epithelial cells. Hepatocytes were not apparent in these neoplasms except within small necrotic regions surrounded by neoplastic biliary epithelial cells. No virus-like particles were observed among the cases examined in this study.
描述了生活在华盛顿州普吉特海湾污染水道中的野生星斑川鲽（Parophyrs vetulus）胆管细胞癌的超微结构和组织学特征。电子显微镜证实胆管上皮细胞是构成这种肿瘤的主要增殖细胞类型。这些细胞的排列方式多样，从组织良好的多个胆管样结构到排列紊乱的多层低分化胆管上皮细胞片层。在胆管样结构或胆管上皮细胞聚集体之间存在由多层胶原纤维和成纤维细胞组成的纤维性基质，巨噬细胞和各种血细胞类型散布于这些层之间。除了在由肿瘤性胆管上皮细胞包围的小坏死区域内，这些肿瘤中未见到明显的肝细胞。在本研究检查的病例中未观察到病毒样颗粒。
