Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA.
J Virol. 2012 Aug;86(16):8884-9. doi: 10.1128/JVI.00358-12. Epub 2012 Jun 6.
Vesicular stomatitis virus (VSV) is a prototype nonsegmented, negative-sense virus used to examine viral functions of a broad family of viruses, including human pathogens. Here we demonstrate that S(2) VSV, an isolate with a small plaque phenotype compared to other Indiana strain viruses, has a transcription defect resulting in an altered pattern and rapid decline of transcription. The S(2) VSV transcription gradient is dominant over the wild-type transcription in a coinfection. This is the first characterization of an altered gradient of transcription not dependent on RNA template sequence or host response and may provide insight into new approaches to viral attenuation.
水疱性口炎病毒（VSV）是一种非分段、负义病毒的原型，用于研究包括人类病原体在内的广泛病毒家族的病毒功能。在这里，我们证明与其他印第安纳株病毒相比具有小斑块表型的 S(2) VSV 具有转录缺陷，导致转录模式改变和迅速下降。在共感染中，S(2)VSV 的转录梯度占主导地位，超过野生型转录。这是首次对不依赖于 RNA 模板序列或宿主反应的改变的转录梯度进行特征描述，可能为病毒减毒提供新的思路。
