Department of Oral and Maxillofacial Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Mar;113(3):327-33. doi: 10.1016/j.tripleo.2011.03.026.
Platelet-rich plasma (PRP) is becoming a new application in tissue engineering and a developing area for clinicians and researchers because it is a natural source of growth factors, many of which can accelerate and promote bone regeneration. However, few studies have reported the potentiality of using PRP as a scaffold in bone tissue engineering. The present study investigated the feasibility of using PRP as a scaffold to carry bone marrow stromal cells (BMSCs) to repair calvarial defects in a rabbit model.
The primary cultured BMSCs were divided into 2 groups. One group was induced with dexamethasone and the other was not induced. Full-thickness bone defects of 5-mm diameter (4 defects per calvarium) were created on the calvaria of 10 New Zealand white rabbits. PRP or whole blood was used, respectively to incorporate the induced or uninduced BMSCs. Then, the composites were activated and applied to repair the defects. The samples were harvested 8 weeks later and bone regeneration was assessed grossly and analyzed by radiographic or histologic examination.
Eight weeks after the implantation of the materials, substantial bone regeneration was observed at the calvarial defect restored with PRP incorporating the induced BMSCs. Less new bone formation was observed at the defect implanted with PRP incorporating the uninduced BMSCs. In contrast, no bone regeneration was detected at the defects implanted with the whole blood incorporating BMSCs, whether the BMSCs were induced or not.
PRP can be used as a scaffold to carry in vitro expanded BMSCs to repair a rabbit's calvarial defect, but its inductive ability to BMSCs was limited.
富含血小板的血浆（PRP）正成为组织工程的新应用，也是临床医生和研究人员的一个发展领域，因为它是生长因子的天然来源，其中许多生长因子可以加速和促进骨再生。然而，很少有研究报道将 PRP 用作骨组织工程支架的潜力。本研究探讨了将 PRP 用作支架携带骨髓基质细胞（BMSCs）修复兔颅骨缺损的可行性。
原代培养的 BMSCs 分为 2 组。一组用地塞米松诱导，另一组不诱导。在 10 只新西兰白兔的颅骨上制作直径为 5mm 的全层骨缺损（每颅骨 4 个缺损）。分别使用 PRP 或全血来携带诱导或未诱导的 BMSCs。然后，激活复合材料并应用于修复缺损。8 周后取样，通过大体和影像学或组织学检查评估骨再生情况。
植入材料 8 周后，用 PRP 携带诱导的 BMSCs 修复的颅骨缺损处观察到大量骨再生。用 PRP 携带未诱导的 BMSCs 修复的缺损处新骨形成较少。相比之下，无论 BMSCs 是否被诱导，用全血携带 BMSCs 植入的缺损处均未检测到骨再生。
PRP 可作为支架携带体外扩增的 BMSCs 修复兔颅骨缺损，但对 BMSCs 的诱导能力有限。
Zotero 插件