链霉菌质噬菌体 pFP4 的特性及其进化意义。

Characterization of Streptomyces plasmid-phage pFP4 and its evolutionary implications.

机构信息

Key Laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, People's Republic of China.

出版信息

Plasmid. 2012 Nov;68(3):170-8. doi: 10.1016/j.plasmid.2012.05.004. Epub 2012 Jun 5.

DOI:10.1016/j.plasmid.2012.05.004

PMID:22677103

Abstract

Autonomous-replicating plasmid pFP4 of Streptomyces sp. FR1 isolated from a heavy metal-contaminated land was cloned and sequenced. Surprisingly, the 40,949-bp pFP4 contains a cluster of 20 genes, resembling these chromosome-integrated prophages of Streptomyces sp. SPB78 and Streptomyces scabiei 87.22. Plasmid pFP4 could transfer by conjugation and a replication locus, iteron/repA/repB, was identified. The filtered FR1 culture could infect both FR1 and FR1 cured of pFP4 to form plaques, and also six out of 13 strains from the same land, but failed to form plaques on other seven strains from same source and all ten Streptomyces species from different sources. pFP4 phage particles were observed by transmission electron microscopy. Major structural proteins (capsid, portal and tail, etc.) of pFP4 virions were encoded by twelve pFP4 genes. pFP4 phage DNA contained 3' protruding cohesive ends of 9-nt. Streptomyces pFP4 represents a novel plasmid-phage.

摘要

从重金属污染土地中分离到的链霉菌 sp. FR1 的自主复制质粒 pFP4 被克隆和测序。令人惊讶的是，40949bp 的 pFP4 包含了 20 个基因簇，类似于链霉菌 sp. SPB78 和链霉菌 scabiei 87.22 的这些染色体整合的噬菌体。质粒 pFP4 可以通过接合转移，并且鉴定了一个复制位点，即 iteron/repA/repB。经过过滤的 FR1 培养物可以感染 FR1 和 FR1 去除 pFP4 的细胞形成噬菌斑，也可以感染来自同一土地的 13 株中的 6 株，但不能感染来自同一来源的另外 7 株和来自不同来源的 10 株链霉菌。通过透射电子显微镜观察到了 pFP4 噬菌体颗粒。pFP4 噬菌体的主要结构蛋白（衣壳、门户和尾部等）由十二个 pFP4 基因编码。pFP4 噬菌体 DNA 含有 3'突出的 9-nt 粘性末端。链霉菌 pFP4 代表了一种新型的质粒-噬菌体。