Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Centre for Infection Research (HZI), D-66123 Saarbrücken, Germany.
Anal Biochem. 2012 Sep 1;428(1):28-30. doi: 10.1016/j.ab.2012.05.024. Epub 2012 Jun 4.
Surface plasmon resonance (SPR) as a label-free biosensor technique has become an important tool in drug discovery campaigns during the last couple of years. For good assay performance, it is of high interest to verify the functional activity on the immobilization of the target protein on the chip. This study illustrates the verification of the catalytic activity of the drug target protein PqsD by monitoring substrate conversion as a decrease in SPR signal and product detection by ultra high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS(2)). This assay would be applicable to control surface activity of immobilized ligands.
表面等离子体共振（SPR）作为一种无标记的生物传感器技术，在过去几年的药物发现活动中已成为一种重要的工具。为了获得良好的分析性能，验证目标蛋白在芯片上的固定化后的功能活性非常重要。本研究通过监测底物转化为 SPR 信号的降低以及通过超高效液相色谱-串联质谱（UHPLC-MS(2)）检测产物，说明了对药物靶标蛋白 PqsD 的催化活性的验证。该测定方法可适用于控制固定化配体的表面活性。
