Dudak Fahriye Ceyda, Boyaci Ismail Hakki
Department of Food Engineering, Hacettepe University, Beytepe, Ankara, Turkey.
Biotechnol J. 2009 Jul;4(7):1003-11. doi: 10.1002/biot.200800316.
Surface Plasmon Resonance (SPR) biosensor technology has been successfully used for the detection of various analytes such as proteins, drugs, DNA, and microorganisms. SPR-based immunosensors that coupled with a specific antigen-antibody reaction, have become a promising tool for the quantification of bacteria as it offers sensitive, specific, rapid, and label-free detection. In this paper, we review the important issues in the development of SPR-based immunoassays for bacteria detection, concentrating on instrumentation, surface functionalization, liquid handling, and surface regeneration. In addition, this review touches on the recent advances in SPR biosensing for sensitivity enhancement.
表面等离子体共振（SPR）生物传感器技术已成功用于检测各种分析物，如蛋白质、药物、DNA和微生物。基于SPR的免疫传感器与特定的抗原-抗体反应相结合，已成为一种有前途的细菌定量工具，因为它提供灵敏、特异、快速且无需标记的检测。在本文中，我们综述了基于SPR的细菌检测免疫分析方法开发中的重要问题，重点关注仪器设备、表面功能化、液体处理和表面再生。此外，本综述还涉及了SPR生物传感在提高灵敏度方面的最新进展。
