Department of Ophthalmology and Visual Sciences, University of Utah, Salt Lake City, UT 84132, USA.
Cornea. 2012 Dec;31(12):1468-75. doi: 10.1097/ICO.0b013e3182473356.
To evaluate the effect of glucocorticoid (triamcinolone acetonide injectable suspension) pretreatment on corneal neovascularization, lymphangiogenesis, and inflammation in a murine penetrating keratoplasty (PK) and corneal suture model.
For the PK model, BALB/c mice were used as recipients and C57BL/6 mice were used as donors. A group pretreated with subconjunctival glucocorticoid and a combination of post-subconjunctival and topical glucocorticoids (group I) was compared with two groups that did not receive glucocorticoid pretreatment [one group received a combination of subconjunctival and topical glucocorticoids postoperatively (group II) and the other group received only topical glucocorticoid treatment postoperatively (group III)]. All groups were treated with subconjunctival glucocorticoid on the day of surgery. For the corneal suture model, BALB/c mice were used. A group receiving only pre-suture glucocorticoid treatment (group A) and a group receiving only post-suture glucocorticoid treatment (group C) were compared with a control group that did not receive glucocorticoid therapy (group B). The degree of neovascularization, lymphangiogenesis, and inflammatory infiltration was compared in each of these models.
In the PK model, the group receiving glucocorticoid pretreatment (group I) showed less neovascularization compared with the posttreatment-only groups (group II, P=0.043; group III, P=0.020) and less lymphangiogenesis compared with group III (P=0.005). In the corneal suture model, the glucocorticoid pretreatment group showed a similar level of neovascularization, lymphangiogenesis, and inflammatory infiltration as the posttreatment-only groups (P>0.05).
Glucocorticoid pretreatment before PK decreases neovascularization and lymphangiogenesis compared with posttransplant glucocorticoid treatment alone.
评估糖皮质激素（曲安奈德注射混悬液）预处理对小鼠穿透性角膜移植（PK）和角膜缝线模型中角膜新生血管、淋巴管生成和炎症的影响。
对于 PK 模型，使用 BALB/c 小鼠作为受者，C57BL/6 小鼠作为供者。一组接受结膜下糖皮质激素预处理和结膜下及局部糖皮质激素联合预处理（I 组），与两组未接受糖皮质激素预处理的组[一组术后接受结膜下和局部糖皮质激素联合治疗（II 组），另一组仅术后接受局部糖皮质激素治疗（III 组）]进行比较。所有组在手术当天均接受结膜下糖皮质激素治疗。对于角膜缝线模型，使用 BALB/c 小鼠。一组仅接受缝线前糖皮质激素治疗（A 组），一组仅接受缝线后糖皮质激素治疗（C 组），与未接受糖皮质激素治疗的对照组（B 组）进行比较。比较这些模型中新生血管、淋巴管生成和炎症浸润的程度。
在 PK 模型中，接受糖皮质激素预处理的组（I 组）与仅接受术后治疗的组（II 组，P=0.043；III 组，P=0.020）相比，新生血管形成较少，与 III 组相比，淋巴管生成较少（P=0.005）。在角膜缝线模型中，糖皮质激素预处理组与仅接受术后治疗的组（A 组，P>0.05）的新生血管、淋巴管生成和炎症浸润程度相似。
与单独术后应用糖皮质激素相比，PK 前糖皮质激素预处理可减少新生血管和淋巴管生成。
