[A/维多利亚/35/72（H3N2）流感病毒持续性变异株血凝素的特性]

[The characteristics of the hemagglutinin from persistent variants of the influenza virus A/Victoria/35/72 (H3N2)].

作者信息

Medvedeva M N, Petrov N A, Vasilenko S K, Simanovskaia V K, Golubev D B

出版信息

Vopr Virusol. 1990 Sep-Oct;35(5):374-6.

Abstract

Electrophoresis in polyacrylamide gel of the reference influenza A/Victoria/35/72 (H3N2) virus and its persisting variants (PV) showed that the PV isolated on the 158th day from the moment of persistence modelling (PV158) had mutation in the gene of hemagglutinin (HA). This mutation is manifested by incomplete HA synthesis at 40 degrees C and increase of mobility of the light HA subunit (HA2). Analysis of nucleotide sequence of the greater part of HA gene of PV158 virus revealed 5 nucleotide substitutions four of which were significant. Three substitutions were found in Hal: 219 (Ser----Phe), 220 (Arg----Gly), 226 (Leu----Gln) and one in HA2: 156 (Thr----Asn). The importance of these mutations in the determination of the PV phenotype is discussed.

摘要

对参考甲型流感病毒A/维多利亚/35/72（H3N2）及其持续变异株（PV）进行聚丙烯酰胺凝胶电泳分析表明，从持续建模开始第158天分离出的PV（PV158）在血凝素（HA）基因上发生了突变。这种突变表现为在40摄氏度时HA合成不完全，以及轻HA亚基（HA2）迁移率增加。对PV158病毒HA基因大部分核苷酸序列的分析揭示了5个核苷酸替换，其中4个具有显著性。在HA1中发现3个替换：219（丝氨酸→苯丙氨酸）、220（精氨酸→甘氨酸）、226（亮氨酸→谷氨酰胺），在HA2中发现1个替换：156（苏氨酸→天冬酰胺）。文中讨论了这些突变在确定PV表型中的重要性。