The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho-minami, Tottori, 680-8553, Japan.
Antonie Van Leeuwenhoek. 2012 Nov;102(4):629-41. doi: 10.1007/s10482-012-9758-3. Epub 2012 Jun 8.
During an investigation of Hypocrea/Trichoderma species inhabiting mushroom bedlogs, we found five strains of an undescribed species from a culture collection. These were analyzed using a combined approach, including morphology of holomorph, cultural studies, and phylogenetic analyses of the rRNA gene cluster of the internal transcribed spacer region, translation elongation factor 1-α, and RNA polymerase subunit II gene sequences. Distinctive morphological characters include stromata with green ascospores produced on potato dextrose agar medium, and Gliocladium-like to irregularly Verticillium-like conidiophores. In phylogenetic analyses, this species belongs to the Semiorbis clade, but its morphological characteristics do not match the other members of this clade. Based on morphological observations and phylogenetic analyses, we describe this as a new species, Trichoderma mienum, representing its Hypocrea teleomorph and Trichoderma anamorph.
在对蘑菇床材中栖息的 Hypocrea/Trichoderma 物种进行调查时，我们从一个培养物集中发现了五个未描述的物种的菌株。我们采用综合方法对这些菌株进行了分析，包括同形的形态学、培养研究以及核糖体 RNA 基因簇的内部转录间隔区、翻译延伸因子 1-α 和 RNA 聚合酶亚基 II 基因序列的系统发育分析。独特的形态特征包括在马铃薯葡萄糖琼脂培养基上产生绿色子囊孢子的菌核，以及类似于 Gliocladium 的不规则 Verticillium 样分生孢子梗。在系统发育分析中，该物种属于 Semiorbis 分支，但它的形态特征与该分支的其他成员不匹配。基于形态观察和系统发育分析，我们将其描述为一个新物种，即 Trichoderma mienum，代表其 Hypocrea 同形和 Trichoderma 无性型。
