Chen Kai, Zhuang Wen-Ying
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Curr Microbiol. 2017 Sep;74(9):1049-1060. doi: 10.1007/s00284-017-1282-2. Epub 2017 Jun 19.
Trichoderma is a dominant component of the soil mycoflora. During the field investigations of northern, central, and southwestern China, three new species in the Stromaticum clade were encountered from soil, and named as T. hebeiense, T. sichuanense, and T. verticillatum. Their phylogenetic positions were determined by analyses of the combined two genes: partial sequences of translation elongation factor 1-alpha and the second largest RNA polymerase subunit-encoding genes. Distinctions between the new species and their close relatives were discussed. Trichoderma hebeiense appeared as a separate terminal branch. The species is distinctive by its oblong conidia and aggregated pustules in culture. Trichoderma sichuanense features in concentric colony and produces numerous clean exudates on aerial mycelium in culture. Trichoderma verticillatum is characterized by its verticillium-like synanamorph and production of abundant chlamydospores. In vitro antagonism towards the new species was tested by dual culture technique.
木霉是土壤真菌区系的主要组成部分。在中国北方、中部和西南部的野外调查中，从土壤中发现了层状木霉分支中的三个新物种，分别命名为河北木霉、四川木霉和轮枝木霉。通过对翻译延伸因子1-α的部分序列和编码第二大RNA聚合酶亚基的基因这两个基因的联合分析，确定了它们的系统发育位置。讨论了新物种与其近缘种之间的区别。河北木霉表现为一个单独的末端分支。该物种的独特之处在于其长方形分生孢子和培养物中的聚集脓疱。四川木霉的特征是菌落呈同心状，在培养物的气生菌丝上产生大量清亮的渗出物。轮枝木霉的特征是其类似轮枝菌的准性型和产生大量厚垣孢子。通过双培养技术测试了对新物种的体外拮抗作用。
