Qiao Min, Du Xing, Zhang Zhe, Xu JianPing, Yu ZeFen
School of Life Sciences, Yunnan University, No. 2 North, Kunming, Yunnan, 650091, P. R. China.
Laboratory for Conservation and Utilization of Bio-resources, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
MycoKeys. 2018 Dec 14(44):63-80. doi: 10.3897/mycokeys.44.30295. eCollection 2018.
Fungi in the genus are widely distributed in China, including in Yunnan province. In this study, we report three new soil-inhabiting species in , named as , and . Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the translation elongation factor 1-α encoding gene (tef1) and the gene encoding the second largest nuclear RNA polymerase subunit (rpb2). Our analyses indicated that the three new species showed consistent divergence amongst each other and from other known and closely related species. Amongst the three, and belong to the Viride Clade. Specifically, is related to three species - , and and is characterised by tree-like conidiophores, generally paired branches, curved terminal branches, spindly to fusiform phialides and subglobose to globose conidia. In contrast, morphologically resembles and and is distinguished by its pyramidal conidiophores, ampulliform to tapered phialides, discrete branches and ovoidal, occasionally ellipsoid, smooth-walled conidia. The third new species, , is a new member of the Harzianum Clade and is closely associated with , and but distinguished from them by having smaller, subglobose to globose, thin-walled conidia.
该属真菌在中国广泛分布,包括云南省。在本研究中,我们报告了该属中三个新的土壤栖息物种,分别命名为 、 和 。描述了它们的菌落和菌丝形态,包括无性态特征。对于每个物种,从核糖体DNA的内部转录间隔区(ITS)、翻译延伸因子1-α编码基因(tef1)和编码第二大核RNA聚合酶亚基的基因(rpb2)这三个基因座获得了它们的DNA序列。我们的分析表明,这三个新物种彼此之间以及与其他已知的近缘物种之间均表现出一致的差异。在这三个物种中, 和 属于绿霉分支。具体而言, 与三个物种—— 、 和 相关,其特征为树状分生孢子梗、通常成对的分支、弯曲的末端分支、细长至梭形的瓶梗以及近球形至球形的分生孢子。相比之下, 在形态上类似于 和 ,其区别在于金字塔形的分生孢子梗、壶形至锥形的瓶梗、离散的分支以及卵形、偶尔为椭圆形的光滑壁分生孢子。第三个新物种 是哈茨木霉分支的新成员,与 、 和 密切相关,但与之不同的是其分生孢子较小,为近球形至球形,壁薄。