State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
Ecotoxicology. 2012 Aug;21(6):1625-32. doi: 10.1007/s10646-012-0945-9. Epub 2012 Jun 8.
The effects of polycyclic aromatic hydrocarbon (PAH) (pyrene) on superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase, peroxidase, malondialdehyde (MDA) and proline were studied in leaves, stems and roots of Bruguiera gymnorrhiza. The results showed that the responses of enzymatic and non-enzymatic antioxidants varied significantly among the three tissues studied. The activities of antioxidant enzymes in PAH-treated stems and roots fluctuated in different stress levels compared to the controls, while the antioxidant enzymes such as SOD, APX in leaves increased when stressed by PAH with a significant positive relation between PAH and leaf SOD or APX activity. Low PAH treatments could also stimulate proline in leaves and stems. MDA content was obviously accumulated in stems and roots under PAH stress while decreased in leaves, indicating that the increased antioxidant enzymes in leaves may partly alleviate lipid peroxidation. For pollution monitoring purpose, SOD and APX in leaves may be potential biomarkers of PAH pollution in intertidal estuaries.
研究了多环芳烃（PAH）（芘）对木榄叶片、茎和根中超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、过氧化氢酶、过氧化物酶、丙二醛（MDA）和脯氨酸的影响。结果表明，三种组织中酶和非酶抗氧化剂的反应差异显著。与对照相比，PAH 处理的茎和根中的抗氧化酶活性在不同的胁迫水平下波动，而 SOD、APX 等抗氧化酶在叶片中受到 PAH 胁迫时增加，PAH 与叶片 SOD 或 APX 活性之间呈显著正相关。低浓度 PAH 处理也能刺激叶片和茎中的脯氨酸。PAH 胁迫下茎和根中的 MDA 含量明显积累，而叶片中则减少，表明叶片中抗氧化酶的增加可能部分缓解了脂质过氧化。对于污染监测目的，叶片中的 SOD 和 APX 可能是潮间带河口 PAH 污染的潜在生物标志物。
