Yasutomi Y, Onuma M, Yamamoto M, Kawakami Y, Azuma I
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Zentralbl Veterinarmed B. 1990 Nov;37(9):712-20. doi: 10.1111/j.1439-0450.1990.tb01119.x.
Nocardia rubra cell wall skeleton (N-CWS) was used for immunotherapy to bovine leukemia virus (BLV)-positive cattle with enlarged subcutaneous lymphatic nodules. Electron microscopical observations showed the infiltration of macrophage and T cells around N-CWS treated lesions. Antitumor effect induced by N-CWS was examined in vitro. The maximum cytolytic activity of macrophage was observed, when the cells were incubated with 10 micrograms/ml of N-CWS. Chemiluminescence response of peripheral blood mononuclear cells (PBMC) using N-CWS as stimulant was observed with a high level of activity for a long period, 5 hr. Mitogenic effect of N-CWS to PBMC was observed in the presence of macrophages but not without macrophages. Furthermore, interleukin 2 activity was recognized in supernatant of PBMC cultured with N-CWS. Maximum cytotoxic T lymphocyte response was induced when PBMC were cultured with 0.1 micrograms/ml of N-CWS.
用红色诺卡氏菌细胞壁骨架（N-CWS）对患有皮下淋巴结肿大的牛白血病病毒（BLV）阳性牛进行免疫治疗。电子显微镜观察显示，N-CWS处理的病变周围有巨噬细胞和T细胞浸润。在体外检测了N-CWS诱导的抗肿瘤作用。当细胞与10微克/毫升的N-CWS孵育时，观察到巨噬细胞的最大细胞溶解活性。以N-CWS为刺激物观察外周血单核细胞（PBMC）的化学发光反应，其活性在长达5小时的时间内保持在较高水平。在有巨噬细胞存在的情况下观察到N-CWS对PBMC的促有丝分裂作用，而在无巨噬细胞时则未观察到。此外，在用N-CWS培养的PBMC上清液中检测到白细胞介素2活性。当PBMC与0.1微克/毫升的N-CWS培养时，诱导出最大的细胞毒性T淋巴细胞反应。
