Yasutomi Y, Onuma M, Davis W C, Kawakami Y
Department of Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Nihon Juigaku Zasshi. 1990 Dec;52(6):1205-10. doi: 10.1292/jvms1939.52.1205.
Analysis of surface marker of cells after intratumor injection with Nocardia rubra cell wall skeleton (N-CWS) resulted in gradually increasing percentage of macrophage, Pan T and BoCD4+ cells. Proportion of BoCD8+ cells gradually increased from 4th day and then decreased from 8th day after the injection. Fresh tumor infiltrated cells obtained from lymphatic nodule at 8 days after injection of N-CWS showed cytotoxic activity against bovine leukemia cell line, but this activity decreased with the time of cultivation and no activity could be detected after 14 days cultivation. These cultured cells were injected twice to lymphatic nodule at one week interval for adoptive immunotherapy and found to induce complete regression of nodule after 5 weeks from first injection.
用红色诺卡氏菌细胞壁骨架（N-CWS）进行瘤内注射后对细胞表面标志物的分析结果显示，巨噬细胞、泛T细胞和BoCD4+细胞的百分比逐渐增加。BoCD8+细胞的比例在注射后第4天开始逐渐增加，然后从第8天开始下降。注射N-CWS 8天后从淋巴结获得的新鲜肿瘤浸润细胞对牛白血病细胞系显示出细胞毒性活性，但这种活性随着培养时间的延长而降低，培养14天后未检测到活性。将这些培养细胞以一周的间隔两次注射到淋巴结进行过继免疫治疗，发现从第一次注射后5周开始结节完全消退。
