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环境中病原体鉴定和真菌检测的分子技术。

Molecular techniques for pathogen identification and fungus detection in the environment.

机构信息

Department of Forest Sciences, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada;

出版信息

IMA Fungus. 2011 Dec;2(2):177-89. doi: 10.5598/imafungus.2011.02.02.09. Epub 2011 Nov 18.

DOI:10.5598/imafungus.2011.02.02.09
PMID:22679603
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3359816/
Abstract

Many species of fungi can cause disease in plants, animals and humans. Accurate and robust detection and quantification of fungi is essential for diagnosis, modeling and surveillance. Also direct detection of fungi enables a deeper understanding of natural microbial communities, particularly as a great many fungi are difficult or impossible to cultivate. In the last decade, effective amplification platforms, probe development and various quantitative PCR technologies have revolutionized research on fungal detection and identification. Examples of the latest technology in fungal detection and differentiation are discussed here.

摘要

许多真菌物种可引起植物、动物和人类的疾病。准确、稳健地检测和定量真菌对于诊断、建模和监测至关重要。此外，真菌的直接检测可以使人们更深入地了解自然微生物群落，特别是因为许多真菌难以或无法培养。在过去十年中，有效的扩增平台、探针开发和各种定量 PCR 技术彻底改变了真菌检测和鉴定的研究。本文讨论了真菌检测和区分的最新技术示例。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/7de85d63d471/ima-2-177-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/8e94e1b0245a/ima-2-177-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/d0addec8a87a/ima-2-177-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/46fd0abccda0/ima-2-177-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/c8b0257d0e58/ima-2-177-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/8c8ce736b167/ima-2-177-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/7de85d63d471/ima-2-177-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/8e94e1b0245a/ima-2-177-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/d0addec8a87a/ima-2-177-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/46fd0abccda0/ima-2-177-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/c8b0257d0e58/ima-2-177-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/8c8ce736b167/ima-2-177-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28c8/3359816/7de85d63d471/ima-2-177-g006.jpg

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