Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany.
J Hosp Infect. 2012 Jul;81(3):177-83. doi: 10.1016/j.jhin.2012.02.012. Epub 2012 Jun 8.
Over the past few years, plasma medicine has become an important field in medical science. Cold plasma has proven anti-inflammatory, antimicrobial and antineoplastic effects.
To test the decontamination power of two cold plasma sources [low-temperature atmospheric pressure plasma jet (APPJ) and dielectric barrier discharge plasma (DBD)] in vivo on human fingertips.
After 3, 15, 30, 60, 90, 120, 150, 180, 210 and 240 s of spot treatment with the APPJ and DBD, the log reduction factors (RFs) of physiological (PF) and artificially (AF) contaminated flora (Staphylococcus epidermidis and Micrococcus luteus) were calculated. The bacterial load was determined after counting. Tolerance (paresthesia, pain and heat) was measured using a numerical rating scale.
Both plasma devices led to a significant reduction in PF and AF. The maximum log reduction factors for PF were 1.3 for the DBD at 210 s and 0.8 for the APPJ at 60 s. For AF, the maximum log reduction factors were 1.7 for the DBD at 90 s and 1.4 for the APPJ at 120 s. Treatment with both devices was well tolerated.
Both the APPJ and DBD were highly effective in eradicating PF and AF from the fingertips of healthy volunteers. No plasma-resistant isolates were observed. Cold plasma appears to have potential for skin disinfection. For hand hygiene purposes, plasma exposure times would need to be reduced significantly by technical means.
在过去的几年中，等离子体医学已成为医学科学中的一个重要领域。冷等离子体已被证明具有抗炎、抗菌和抗肿瘤作用。
测试两种冷等离子体源（低温大气压等离子体射流（APPJ）和介质阻挡放电等离子体（DBD））在人体指尖上的体内去污能力。
用 APPJ 和 DBD 进行 3、15、30、60、90、120、150、180、210 和 240 s 的点处理后，计算生理（PF）和人工（AF）污染菌群（表皮葡萄球菌和微球菌）的对数减少因子（RF）。通过计数确定细菌负荷。使用数字评分量表测量耐受性（感觉异常、疼痛和发热）。
两种等离子体装置均导致 PF 和 AF 显著减少。DBD 在 210 s 时对 PF 的最大对数减少因子为 1.3，而 APPJ 在 60 s 时为 0.8。对于 AF，DBD 在 90 s 时的最大对数减少因子为 1.7，而 APPJ 在 120 s 时为 1.4。两种装置的治疗均耐受良好。
APPJ 和 DBD 均能有效消除健康志愿者指尖上的 PF 和 AF。未观察到对等离子体有抗性的分离株。冷等离子体似乎具有皮肤消毒的潜力。为了手部卫生目的，需要通过技术手段显著减少等离子体暴露时间。
