Division of Hematology, Department of Medicine, Nippon Medical School, Tokyo, Japan.
Leuk Res. 2012 Oct;36(10):1229-36. doi: 10.1016/j.leukres.2012.05.006. Epub 2012 Jun 7.
We conducted a multicenter, flow cytometry study to validate differences in immunophenotypes among disease types in melodysplastic syndromes (MDS). The data obtained from 115 patients were combined into three groups according to disease grade, i.e., low-grade MDS, refractory anemia with excess blasts, and acute leukemia transformed from MDS (AL-MDS). The data comparison showed that with the progression of disease grade, the immunophenotypes of CD34(+) myeloblasts were more immature, with an increase and a decrease in CD7 and CD15 expression, respectively, and the percentages of CD34(+) B-progenitors among total CD34(+) cells and the granularity of granulocytes decreased. Logistic regression analyses showed that, in addition to myeloblast percentages, the expression of CD7 and B7-H1 on myeloblasts was independently associated with AL-MDS patients.
我们进行了一项多中心流式细胞术研究，以验证骨髓增生异常综合征（MDS）中不同疾病类型之间免疫表型的差异。将 115 例患者的数据合并为三组，根据疾病分级，即低级别 MDS、伴原始细胞增多的难治性贫血和 MDS 转化的急性髓系白血病（AL-MDS）。数据比较显示，随着疾病分级的进展，CD34+原始细胞的免疫表型变得更加不成熟，CD7 和 CD15 的表达分别增加和减少，总 CD34+细胞中的 CD34+B 祖细胞比例和粒细胞的粒度也降低。逻辑回归分析表明，除原始细胞百分比外，原始细胞上 CD7 和 B7-H1 的表达与 AL-MDS 患者独立相关。
