组氨酸与多巴脱羧酶的免疫交叉反应性。

Immuno-cross-reactivity of histidine and dopa decarboxylases.

作者信息

Mizuguchi H, Yabumoto M, Imamura I, Fukui H, Wada H

机构信息

Department of Pharmacology II, Osaka University Faculty of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Dec 31;173(3):1299-303. doi: 10.1016/s0006-291x(05)80928-3.

DOI:10.1016/s0006-291x(05)80928-3

PMID:2268331

Abstract

Immuno-cross-reactivity between histidine decarboxylase (HDC) and dopa decarboxylase (DDC) was investigated. By comparing the cDNA sequences of rat HDC with rat and guinea-pig DDCs, we found a region that may possibly be related to the cross-reactivity of anti-rat HDC antibody with guinea-pig DDC. The peptide encoded by this region was synthesized and anti-peptide antibody was prepared. We also purified HDC and DDC homogeniously from fetal rat liver and guinea-pig liver, respectively. On immunoblotting, anti-peptide antibody recognized both rat HDC and guinea-pig DDC. Anti-HDC polyclonal antibody which also recognizes both enzymes detected only rat HDC when it was absorbed by the peptide. This result indicates that this region is responsible for the immuno-cross-reactivity of anti-rat HDC antibody with guinea-pig DDC.

摘要

研究了组氨酸脱羧酶（HDC）与多巴脱羧酶（DDC）之间的免疫交叉反应性。通过比较大鼠HDC与大鼠和豚鼠DDC的cDNA序列，我们发现了一个可能与抗大鼠HDC抗体与豚鼠DDC交叉反应性相关的区域。合成了该区域编码的肽并制备了抗肽抗体。我们还分别从胎鼠肝脏和豚鼠肝脏中纯化了HDC和DDC。在免疫印迹中，抗肽抗体识别大鼠HDC和豚鼠DDC。同时识别这两种酶的抗HDC多克隆抗体在被该肽吸收后仅检测到大鼠HDC。该结果表明该区域负责抗大鼠HDC抗体与豚鼠DDC的免疫交叉反应性。