抗大鼠组氨酸脱羧酶抗体与豚鼠多巴脱羧酶交叉反应的免疫组织化学分析。
Immunohistochemical analysis of the cross-reaction of anti-rat histidine decarboxylase antibody with guinea-pig DOPA decarboxylase.
作者信息
Taguchi Y, Watanabe T, Shiosaka S, Tohyama M, Wada H
出版信息
Brain Res. 1985 Aug 12;340(2):235-42. doi: 10.1016/0006-8993(85)90919-9.
L-Histidine decarboxylase [L-histidine carboxylyase, HDC, EC 4.1.1.22] is an enzyme distinct from L-DOPA decarboxylase [L-aromatic amino acid carboxylyase, DDC, EC 4.1.1.28]: the two decarboxylases from fetal rat liver were completely separated from each other by DEAE-cellulose column chromatography and by affinity chromatography with L-carnosine as a ligand. The antibody raised against this HDC inhibited the HDC's from rat and guinea-pig brains very strongly, but their DDCs very weakly. However, in immunofluorescent histochemical studies, the antibody cross-reacted with DDC-like immunoreactive structures, such as chromaffin cells of the adrenal medulla, the raphe nucleus, the substantia nigra, and the locus coeruleus of the brain of guinea-pigs, but not of rats, suggesting that these two decarboxylases share some antigenic structures.
L-组氨酸脱羧酶[L-组氨酸羧化酶，HDC，EC 4.1.1.22]是一种与L-多巴脱羧酶[L-芳香族氨基酸羧化酶，DDC，EC 4.1.1.28]不同的酶：通过DEAE-纤维素柱色谱法以及以L-肌肽作为配体的亲和色谱法，可将来自胎鼠肝脏的这两种脱羧酶完全彼此分离。针对这种HDC产生的抗体对大鼠和豚鼠脑内的HDC具有非常强的抑制作用，但对它们的DDC抑制作用非常弱。然而，在免疫荧光组织化学研究中，该抗体与DDC样免疫反应性结构发生交叉反应，如豚鼠脑而非大鼠脑的肾上腺髓质嗜铬细胞、中缝核、黑质和蓝斑，但这表明这两种脱羧酶具有一些共同的抗原结构。
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