Reinlib L, Akinshola E, Potter J J, Mezey E
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Biochem Biophys Res Commun. 1990 Dec 31;173(3):774-80. doi: 10.1016/s0006-291x(05)80854-x.
Rat hepatocytes were studied for [Ca2+]i with Fura-2 at the single cell level using a microfluorometer-imaging system which showed that both the number of cells elevating [Ca2+]i and the magnitude of [Ca2+]i increase were directly dependent upon ethanol concentration between 50 mM and 1 M. Peak [Ca2+]i increases ranged from 27 nM with 50 mM ethanol to 57 nM after 1 M ethanol. Ethanol appeared to initiate calcium release from intracellular stores and caused a dose dependent production of inositol(1,4,5) triphosphate (Ins(1,4,5)P3) in hepatocytes. Low concentrations of ethanol (50-100 mM) did not significantly raise Ins(1,4,5)P3 although 300 mM-1 M increased Ins(1,4,5)P3 comparable to that found with vasopressin (5 nM). In summary, physiologic amounts of ethanol raise [Ca2+]i in rat hepatocytes, although at lower levels (50-100 mM) the changes may or may not be related to an Ins(1,4,5)P3 pathway.
使用显微荧光计成像系统在单细胞水平上用Fura-2研究大鼠肝细胞的[Ca2+]i，结果表明，细胞内[Ca2+]i升高的细胞数量以及[Ca2+]i升高的幅度均直接取决于乙醇浓度，乙醇浓度范围在50 mM至1 M之间。[Ca2+]i的峰值升高范围从50 mM乙醇时的27 nM到1 M乙醇后的57 nM。乙醇似乎引发了细胞内钙库的钙释放，并导致肝细胞中肌醇（1,4,5）三磷酸（Ins(1,4,5)P3）的剂量依赖性产生。低浓度乙醇（50 - 100 mM）不会显著提高Ins(1,4,5)P3，尽管300 mM - 1 M乙醇使Ins(1,4,5)P3升高的幅度与血管加压素（5 nM）相当。总之，生理量的乙醇会使大鼠肝细胞内的[Ca2+]i升高，尽管在较低浓度（50 - 100 mM）时，这种变化可能与Ins(1,4,5)P3途径有关，也可能无关。
