Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
Environ Toxicol Pharmacol. 2012 Sep;34(2):324-333. doi: 10.1016/j.etap.2012.04.011. Epub 2012 May 3.
Microcystin-LR (MCLR) is a widespread cyanotoxin produced in algal blooms, and has potent hepatotoxicity and tumor-promoting activity. We cloned the full-length cDNA of Id1 in bighead carp. The full-length Id1 cDNA was 954bp and contained a 387bp ORF. Bighead carp Id1 shared high identity with zebrafish Id1 amino acid sequence, and phylogenetic analysis showed that teleost Id1 evolved closely. Bighead carp Id1 constitutively expressed in all tested tissues in normal. When tested at two different time points post exposure and at 3 different MCLR doses, Id1 expression increased in a time-dependent pattern, and Id1 expression in brain was very sensitive to MCLR exposure. The present study will help us to understand more about the evolution of Id1 molecule and its role in the MCLR induced cell differentiation and cancer promoting in bighead carp.
微囊藻毒素-LR（MCLR）是一种广泛存在于藻类水华中的蓝藻毒素，具有强烈的肝毒性和肿瘤促进活性。我们在大头鱼中克隆了 Id1 的全长 cDNA。Id1 的全长 cDNA 为 954bp，包含一个 387bp 的 ORF。大头鱼 Id1 与斑马鱼 Id1 氨基酸序列具有高度的同源性，系统进化分析表明硬骨鱼 Id1 进化关系密切。Id1 在正常大头鱼的所有检测组织中均持续表达。在暴露于不同时间点和 3 种不同 MCLR 剂量后的检测中，Id1 的表达呈时间依赖性增加，并且大脑中的 Id1 对 MCLR 暴露非常敏感。本研究将帮助我们更多地了解 Id1 分子的进化及其在大头鱼 MCLR 诱导的细胞分化和癌症促进中的作用。
