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鉴定和表达谱的白细胞介素-8 在大头鱼 （鳙鱼） 对微囊藻毒素-LR 的反应。

Identification and expression profiles of IL-8 in bighead carp (Aristichthys nobilis) in response to microcystin-LR.

机构信息

Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China.

出版信息

Arch Environ Contam Toxicol. 2013 Oct;65(3):537-45. doi: 10.1007/s00244-013-9910-8. Epub 2013 Jun 25.

DOI:10.1007/s00244-013-9910-8
PMID:23797979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3763166/
Abstract

Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.

摘要

微囊藻毒素-LR（MCLR）是一种广泛存在的蓝藻毒素，对动物具有免疫毒性，包括鱼类。趋化因子被认为在 MCLR 诱导的炎症反应中发挥重要作用。在这项研究中，我们首次从大头鱼（Aristichthys nobilis）中克隆了白细胞介素-8（IL-8）的全长 cDNA。IL-8 cDNA 全长 552bp，包含一个编码 98 个氨基酸的蛋白质的 297bp 开放阅读框。推导的 IL-8 蛋白在 N 端具有典型的天冬氨酸（D）-亮氨酸（L）-精氨酸（R）和 CXC 基序，在大多数鱼类中都保守。系统发育分析表明，大头鱼 IL-8 蛋白属于硬骨鱼 IL-8 谱系 2。在正常情况下，IL-8 在所有检测组织中均呈组成型表达且较弱。然而，MCLR 处理以时间和剂量依赖的方式显著增加大头鱼 IL-8 的转录。本研究将帮助我们更多地了解 IL-8 的进化及其在大头鱼 MCLR 诱导的促炎反应中的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/06a294e6b3ba/244_2013_9910_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/6329aa5eff93/244_2013_9910_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/3b0c911d9d6d/244_2013_9910_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/1450b16757fb/244_2013_9910_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/a2fed49a5aa9/244_2013_9910_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/06a294e6b3ba/244_2013_9910_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/6329aa5eff93/244_2013_9910_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/3b0c911d9d6d/244_2013_9910_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/1450b16757fb/244_2013_9910_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/a2fed49a5aa9/244_2013_9910_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3496/3763166/06a294e6b3ba/244_2013_9910_Fig5_HTML.jpg

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