von Zglinicki T, Uerlings I
Microsc Acta. 1979 Jul;82(1):69-75.
DNA is adsorbed with densities sufficient for electron microscopy on thin uncharged carbon films. Superhelical SV 40 DNA remains superhelical on the supporting film on the contrary to the relaxation of DNA on films treated with alky dimethylbenzyl ammonium chloride (BAC). The number of superhelical turns increases to the two- or threefold of the value in solution. Investigations with a structural resolution below 1nm are possible with this preparation method.
DNA以足以用于电子显微镜观察的密度吸附在薄的不带电碳膜上。与用烷基二甲基苄基氯化铵（BAC）处理的膜上DNA的松弛情况相反，超螺旋SV 40 DNA在支撑膜上仍保持超螺旋状态。超螺旋匝数增加到溶液中值的两到三倍。用这种制备方法可以进行结构分辨率低于1nm的研究。
