Wu Shiwu, Yu Lan, Cheng Zenong, Song Wenqing, Zhou Lei, Tao Yisheng
Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu, 233000, China.
J Huazhong Univ Sci Technolog Med Sci. 2012 Jun;32(3):346-352. doi: 10.1007/s11596-012-0060-4. Epub 2012 Jun 9.
Maspin belongs to the serine protease inhibitor (serpin) family and has been proven to be a suppressor of tumor growth and metastasis in many types of tumors. The purpose of this study was to investigate the expression of maspin in non-small cell lung cancer (NSCLC) and its relationship to vasculogenic mimicry (VM). A total of 160 specimens of NSCLC were involved in this study and 20 specimens of normal lung tissue served as controls. VM, microvessel density (MVD) and the expression of maspin were detected by using immunohistochemical staining. The results showed that the positive rates of maspin and VM in the NSCLC group were 48.1% (77/160) and 36.9% (59/160), respectively, which were significantly different from those in the control group with the positive rates of maspin and VM being 100% and 0% respectively (P<0.05). VM, MVD and the expression level of maspin were significantly related to tumor differentiation, lymph node metastasis, clinical stages and postoperative survival time (all P<0.05). The maspin expression in patients with squamous cell carcinoma was significantly higher than that in those with adenocarcinoma (P<0.05). The maspin expression was negatively correlated with VM and MVD, and there was a positive correlation between VM and MVD. Maspin-negative expression, VM and high MVD score were negatively related to the 5-year-survival rate. PTNM stages, VM, MVD and maspin expression were independent prognostic factors for NSCLC (P<0.05). It was suggested that the loss of expression of maspin may participate in the invasion and metastasis of NSCLC and it has a positive relationship to VM in NSCLC. Combined detection of maspin, VM and MVD may help predict the progression and prognosis of NSCLC.
Maspin属于丝氨酸蛋白酶抑制剂（serpin）家族，并且已被证实在多种类型的肿瘤中是肿瘤生长和转移的抑制因子。本研究的目的是探讨maspin在非小细胞肺癌（NSCLC）中的表达及其与血管生成拟态（VM）的关系。本研究共纳入160例NSCLC标本，20例正常肺组织标本作为对照。采用免疫组织化学染色检测VM、微血管密度（MVD）和maspin的表达。结果显示，NSCLC组maspin和VM的阳性率分别为48.1%（77/160）和36.9%（59/160），与对照组相比差异有统计学意义，对照组maspin和VM的阳性率分别为100%和0%（P<0.05）。VM、MVD和maspin的表达水平与肿瘤分化、淋巴结转移、临床分期及术后生存时间均显著相关（均P<0.05）。鳞状细胞癌患者的maspin表达明显高于腺癌患者（P<0.05）。maspin表达与VM和MVD呈负相关，VM和MVD之间呈正相关。Maspin阴性表达、VM和高MVD评分与5年生存率呈负相关。PTNM分期、VM、MVD和maspin表达是NSCLC的独立预后因素（P<0.05）。提示maspin表达缺失可能参与NSCLC的侵袭和转移，且与NSCLC中的VM呈正相关。联合检测maspin、VM和MVD可能有助于预测NSCLC的进展和预后。
