Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, 27 Shanda Nanlu, Jinan, 250100 Shandong, People's Republic of China.
Environ Sci Pollut Res Int. 2013 Feb;20(2):1059-69. doi: 10.1007/s11356-012-1018-6. Epub 2012 Jun 10.
Wastewater treatment is an important source of nitrous oxide (N(2)O), which is a strong greenhouse gas and dominate ozone-depleting substance. The purpose of this study was to evaluate the effect of carbon source on N(2)O emission from anoxic/oxic biological nitrogen removal process. The mechanisms of N(2)O emission were also studied. Long-term experiments were operated to evaluate the effect of three different carbon sources (i.e., glucose, sodium acetate, and soluble starch) on N(2)O emission characteristics. And batch experiments, in the presence or absence of specific inhibitors, were carried out to identify the sources of N(2)O emission. The ammonia-oxidizing bacteria (AOB) and denitrifiers community compositions under different circumstances were also analyzed based on which the underlying mechanisms of N(2)O emission were elucidated. The conversion ratios of N(2)O in reactors with glucose, sodium acetate, and soluble starch were 5.3 %, 8.8 %, and 2.8 %, respectively. The primary process responsible for N(2)O emission was nitrifier denitrification by Nitrosomonas-like AOB, while denitrification by heterotrophic denitrifiers acted as the sink. Reactor with sodium acetate showed the highest N(2)O emission, together with the highest nitrogen and phosphate removal ratios. Carbon source has a significant impact on N(2)O emission quantity and relatively minor effect on its production mechanism.
污水处理是产生氧化亚氮（N₂O）的重要来源，氧化亚氮是一种强温室气体，也是消耗臭氧物质的主要成分。本研究旨在评估不同碳源对缺氧/好氧生物脱氮工艺中 N₂O 排放的影响，并对 N₂O 排放机制进行研究。通过长期实验评估了三种不同碳源（葡萄糖、乙酸钠和可溶性淀粉）对 N₂O 排放特性的影响。同时进行了批式实验，在添加或不添加特定抑制剂的情况下，以确定 N₂O 排放的来源。此外，还根据不同情况下氨氧化细菌（AOB）和反硝化菌群落组成，对 N₂O 排放的潜在机制进行了分析。结果表明，在含有葡萄糖、乙酸钠和可溶性淀粉的反应器中，N₂O 的转化率分别为 5.3%、8.8%和 2.8%。N₂O 排放的主要过程是亚硝化菌主导的硝化反硝化，而异养反硝化菌则作为 N₂O 的汇。含有乙酸钠的反应器中，N₂O 排放量最高，同时氮和磷的去除率也最高。碳源对 N₂O 排放量有显著影响，而对其产生机制的影响相对较小。
