Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research, Madrid, Spain.
Proteomics. 2012 Aug;12(14):2331-9. doi: 10.1002/pmic.201100588.
We used a brief trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS to identify the surface proteins on live Candida albicans organisms growing in biofilms and planktonic yeast cells and hyphae. One hundred thirty-one proteins were present in at least two of the three replicates of one condition and distributed in various combinations of the three growth conditions. Both previously reported and new surface proteins were identified and these were distributed between covalently attached proteins and noncovalently attached proteins of the cell wall.
我们采用短暂的胰酶处理，然后通过纳升液相色谱分离和鉴定肽段，再结合离线 MS/MS，来鉴定生物膜和浮游酵母细胞及菌丝中活体白色念珠菌的表面蛋白。在至少两种条件的三个重复中，有 131 种蛋白质存在，且分布在三种生长条件的各种组合中。鉴定出了既有报道的也有新的表面蛋白，这些蛋白分布在细胞壁的共价结合蛋白和非共价结合蛋白之间。
