凝血酶受体表达对食管鳞癌细胞微血管内皮细胞和生存状态的影响与凝血状态的关系。

Effect of thrombin receptor expression on microvessel endothelial cells, and survival by coagulation state in oesophageal squamous cell carcinoma.

机构信息

Women's Hospital School of medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

出版信息

J Clin Pathol. 2012 Aug;65(8):740-5. doi: 10.1136/jclinpath-2011-200568. Epub 2012 Jun 9.

DOI:10.1136/jclinpath-2011-200568

PMID:22685255

Abstract

PURPOSE

The authors aimed to evaluate the prognostic value of thrombin receptor (TR) expression in microvessel endothelial cells (VECs) by coagulation state in oesophageal squamous cell carcinoma (ESCC).

METHODS

In 138 ESCC normal and para-tumour samples, we investigated the association of the expression of TR and CD34 (microvessel marker) seen on immunohistochemical staining and clinicopathological parameters, coagulation state, microvessel density (MVD), and survival of patients.

RESULT

In total, 62 ESCC tissues were positive for TR in VECs, and in 48 cases, the activated partial thromboplastin time (APTT) was <28.5 s, with significant difference in tumour depth between TR-positive and -negative cases with APTT<28.5 s. Also with APTT<28.5 s, TR-positive MVD (TRMVD) was significantly greater with high than low CD34-positive MVD (CD34MVD; 7.96±4.27 vs 5.64±2.99; p=0.032). High CD34MVD was associated with increased TR expression in patients with APTT<28.5 than ≥28.5 s. In patients with high CD34MVD, the number of TR-positive microvessels was greater with APTT<28.5 than ≥28.5 s (n=17 vs n=6; p=0.002), and APTT and TR expressions were negatively correlated for patients with APTT<28.5 s (r(2) =-0.472, p=0.023). For patients with APTT<28.5 s, the cumulative survival rate was poorer with high than low TRMVD (p=0.027). On multivariate analysis, tumour size (p=0.006), tumour stage (p=0.004) and TRMVD (p=0.024) were independently associated with survival for patients with APTT<28.5 s. TRMVD had the second highest HR.

CONCLUSION

TR positivity in VECs may be an adverse prognostic factor for patients with ESCC and increased coagulation state. TR expression in VECs might be related to angiogenesis in ESCC.

摘要

目的

作者旨在通过凝血状态评估血栓酶受体（TR）在食管鳞状细胞癌（ESCC）微血管内皮细胞（VEC）中的表达对预后的影响。

方法

在 138 例 ESCC 正常和肿瘤旁样本中，我们通过免疫组织化学染色检测了 TR 和 CD34（微血管标志物）的表达与临床病理参数、凝血状态、微血管密度（MVD）和患者生存之间的关系。

结果

在总共 62 例 ESCC 组织中，VEC 中的 TR 呈阳性，在 48 例病例中，活化部分凝血活酶时间（APTT）<28.5 s，TR 阳性病例与 APTT<28.5 s 的肿瘤深度有显著差异。此外，APTT<28.5 s 时，TR 阳性 MVD（TRMVD）与高 CD34 阳性 MVD（CD34MVD）相比显著增加（7.96±4.27 vs 5.64±2.99；p=0.032）。APTT<28.5 s 时，CD34MVD 高的患者中 TR 表达增加。在 CD34MVD 高的患者中，APTT<28.5 s 时 TR 阳性微血管数量大于 APTT≥28.5 s 时（n=17 对 n=6；p=0.002），APTT 和 TR 表达呈负相关（r²=-0.472，p=0.023）。对于 APTT<28.5 s 的患者，高 TRMVD 组的累积生存率低于低 TRMVD 组（p=0.027）。多因素分析显示，APTT<28.5 s 的患者中，肿瘤大小（p=0.006）、肿瘤分期（p=0.004）和 TRMVD（p=0.024）与生存独立相关。TRMVD 的 HR 居第二位。