Ameni Gobena, Cockle Paul, Lyashchenko Konstantin, Vordermeier Martin
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia.
Vet Med Int. 2012;2012:457872. doi: 10.1155/2012/457872. Epub 2012 May 20.
Higher IFN-γ responses to mycobacterial antigens were observed in Bos taurus (Holsteins) than in Bos indicus (Zebu) cattle which could due to differences in antigen recognition profiles between the two breeds. The present study was conducted to evaluate mycobacterial antigen recognition profiles of the two breeds. Twenty-three mycobacterial antigens were tested on 46 skin test positive (24 Zebu and 22 Holstein) using enzyme-linked immunospot assay (ELISPOT) and multiple antigen print immunoassay (MAPIA). Herds from which the study cattle obtained were tested for Fasciola antibody. The T cells from both breeds recognized most of the mycobacterial antigens at lower and comparable frequencies. However, antigens such as CFP-10, ESAT-6, Rv0287, Rv0288, MPB87, Acr-2, Rv3616c, and Rv3879c were recognized at higher frequencies in zebu while higher frequencies of T cell responses were observed to Hsp65 in both breeds. Furthermore, comparable antibody responses were observed in both breeds; MPB83 being the sero-dominant antigen in both breeds. The prevalence of Fasciola antibody was 81% and similar in both breeds. This piece of work could not lead to a definitive conclusion if there are differences in mycobacterial recognition profiles between the two breeds warranting for further similar studies using sound sample size from the two breeds.
与瘤牛（泽布牛）相比，在普通牛（荷斯坦牛）中观察到对分枝杆菌抗原的γ干扰素反应更高，这可能是由于两个品种之间抗原识别谱的差异所致。本研究旨在评估这两个品种的分枝杆菌抗原识别谱。使用酶联免疫斑点测定法（ELISPOT）和多重抗原印迹免疫测定法（MAPIA），对46头皮肤试验呈阳性的牛（24头泽布牛和22头荷斯坦牛）检测了23种分枝杆菌抗原。对提供研究用牛的牛群进行了肝片吸虫抗体检测。两个品种的T细胞以较低且相当的频率识别大多数分枝杆菌抗原。然而，诸如CFP-10、ESAT-6、Rv0287、Rv0288、MPB87、Acr-2、Rv3616c和Rv3879c等抗原在泽布牛中被识别的频率更高，而在两个品种中对Hsp65的T细胞反应频率都较高。此外，在两个品种中观察到了相当的抗体反应；MPB83是两个品种中的血清优势抗原。肝片吸虫抗体的流行率为81%，在两个品种中相似。如果两个品种之间在分枝杆菌识别谱上存在差异，这项工作无法得出明确结论，需要使用来自两个品种的合理样本量进行进一步的类似研究。
